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SE Cell Line 4D X Kit L Amaxa®Nucleofector®技术是 Lonza(原Amaxa)公司的专利创新技术，它综合应用传统的电穿孔技术及细胞特异性细胞核转染液，通过调整优化的电转参数（内存转染程序），直接把外源基因导入原代细胞及细胞系的细胞浆和细胞核中。它不同于传统的电穿孔仪，因为它可以把外源基因直接导入核中，而且细胞存活率远远高于电穿孔仪。 SE Cell Line 4D X Kit L适用于4D-Nucleofector细胞核转染系统，该系统采用导电性聚合物，取代了金属铝离子，保证高效转染的同时获得高的细胞存活率。专利的导电性聚合物材料替代传统的金属铝离子电极；克服传统金属电极释放的金属离子（Al3+对细胞的毒性）对转染效果的影响；提高（或维持）转染效率的同时，保证细胞的生理特性。 试剂盒组分 1. 细胞特异性核转染液（SE） 2. 添加剂 3 .pmaxGFP™ 阳性质粒 4. 单孔电转杯（100μl）或16孔电转板条（20μl） 产品优势 1）每种细胞转染液（Nucleofector™ Solutions）都可以用于筛选不同的细胞系； 2）根据使用平台优化Nucleofector™转染条件。 Nucleofector 细胞核转染技术操作步骤（详细Protocol请登录Lonza官方网站查阅）
整个操作过程只需四步，非常简单、高效。
第1步：处理细胞，精确计数
第2步：混合DNA、转染液，重悬细胞，加入转染杯
第3步：将转染杯放入转染仪，按键选择相应程序，按"start"键开始转染，10秒钟左右
第4步：取出细胞继续培养。Material SAPMaterial Prod.hier. Productgroup UOM MaterialDescription VHNP-1001 00193552 CF3952H AmaxaNFreag PC 96-wellNucleocuvette™Plate VS4-00500P 00201528 CF3950T AmaxaNFreag PC Nucleofector™PLUS-4Supplement,500µl V4YP-2A24 00199802 CF3950S AmaxaNFreag EA AD24D-Nucleofector™YKit V4YP-9A48 00199803 CF3950S AmaxaNFreag EA PrimaryCellOpt.4D-Nucleofector™YKit V4XC-3012 00197169 CF3950L AmaxaNFreag KT SGCellLine4DXKitL(12RCT) VAPB-1001 00193076 CF3950J AmaxaNFreag PC 10RCTHCAECKit VAPB-1003 00193078 CF3950J AmaxaNFreag PC 10RCTHMVEC-LKit V4YP-1A24 00199801 CF3950S AmaxaNFreag EA AD14D-Nucleofector™YKit VS3-00150P 00201526 CF3950T AmaxaNFreag PC Nucleofector™PLUS-3Supplement,150µl VAPA-1010 00193074 CF3950J AmaxaNFreag PC 10RCTMouseBCellKit VAPK-1002 00193106 CF3950J AmaxaNFreag PC 10RCTHMECKit V4XP-5012 00197181 CF3950P AmaxaNFreag KT P5PrimaryCell4DXKitL(12RCT) VAMI-1021 00193066 CF3950J AmaxaNFreag PC 10RCTBasicParasiteKit2 V4XC-2012 00197168 CF3950L AmaxaNFreag KT SFCellLine4DXKitL(12RCT) V4XP-2012 00197178 CF3950P AmaxaNFreag KT P2PrimaryCell4DXKitL(12RCT) V4XP-4012 00197180 CF3950P AmaxaNFreag KT P4PrimaryCell4DXKitL(12RCT) V4XP-1012 00197177 CF3950P AmaxaNFreag KT P1PrimaryCell4DXKitL(12RCT) V4XC-1012 00197167 CF3950L AmaxaNFreag KT SECellLine4DXKitL(12RCT) VAPA-1005 00193071 CF3950J AmaxaNFreag PC 10RCTHumanNKCellKit VAPC-1001 00193080 CF3950J AmaxaNFreag PC 10RCTHumanAoSMCKit VAPD-1003 00193083 CF3950J AmaxaNFreag PC 10RCTNHEM-NeoKit VAPF-1001 00193088 CF3950J AmaxaNFreag PC 10RCTHumanChondrocyteKit VAPD-1002 00193082 CF3950J AmaxaNFreag PC 10RCTHumanKeratinocyteKit VAPE-1002 00193087 CF3950J AmaxaNFreag PC 10RCTRatCardiomyocyteNeoKit VAPG-1002 00193090 CF3950J AmaxaNFreag PC 10RCTChickenNeuronKit V4XP-3012 00197179 CF3950P AmaxaNFreag KT P3PrimaryCell4DXKitL(12RCT) VAMI-1011 00193065 CF3950J AmaxaNFreag PC 10RCTBasicParasiteKit1 V4XP-2024 00197183 CF3950Q AmaxaNFreag KT P2PrimaryCell4DXKitL(24RCT) V4XP-5024 00197187 CF3950Q AmaxaNFreag KT P5PrimaryCell4DXKitL(24RCT) VS3-00500P 00201527 CF3950T AmaxaNFreag PC Nucleofector™PLUS-3Supplement,500µl V4XP-4024 00197186 CF3950Q AmaxaNFreag KT P4PrimaryCell4DXKitL(24RCT) V4XP-1024 00197182 CF3950Q AmaxaNFreag KT P1PrimaryCell4DXKitL(24RCT) VAPB-1002 00193077 CF3950J AmaxaNFreag PC 10RCTHUVECKit V4XC-3024 00197172 CF3950M AmaxaNFreag KT SGCellLine4DXKitL(24RCT) V4XC-1024 00197170 CF3950M AmaxaNFreag KT SECellLine4DXKitL(24RCT) VAPA-1008 00193072 CF3950J AmaxaNFreag PC 10RCTHumanMacrophageKit VAPA-1009 00193073 CF3950J AmaxaNFreag PC 10RCTMouseMacrophageKit V4XP-3024 00197184 CF3950Q AmaxaNFreag KT P3PrimaryCell4DXKitL(24RCT) V4XC-2024 00197171 CF3950M AmaxaNFreag KT SFCellLine4DXKitL(24RCT) VECA-1001 00192752 CF3956B AmaxaNFreag PC CLB-Transfection™Kit V5SP-9001 00199450 CF3952M AmaxaNFreag PC PrimaryCellOptimiz.384-wellKit(1PT) V4XP-1A32 00197194 CF3950S AmaxaNFreag PC BasicNeuron4DXADKit(32RCT) VACA-1004 00193063 CF3950H AmaxaNFreag PC 10RCTCellLineKitC V5SC-9001 00199462 CF3952L AmaxaNFreag PC CellLineOptimization384-wellKit VMI-1001 00191767 CF3950D AmaxaNFreag PC BasicParasiteNucleofector®StarterKit VAPA-1011 00193075 CF3950J AmaxaNFreag PC 10RCTMouseDendriticCellKit VAPL-1004 00198769 CF3950J AmaxaNFreag PC 10RCTMouse/RatHepatocyteKit VAPH-5022 00193099 CF3950J AmaxaNFreag PC 10RCTHumanStemCellKit2 VAPD-1005 00193085 CF3950J AmaxaNFreag PC 10RCTMEF2Kit VAPE-1001 00193086 CF3950J AmaxaNFreag PC 10RCTHumanMSCKit VAPI-1006 00198767 CF3950J AmaxaNFreag PC 10RCTBasicGlialCellsKit V4XP-3032 00197190 CF3950R AmaxaNFreag KT P3PrimaryCell4DXKitS(32RCT) VAPH-5012 00193098 CF3950J AmaxaNFreag PC 10RCTHumanStemCellKit1 VAPA-1001 00193067 CF3950J AmaxaNFreag PC 10RCTHumanBCellKit VAPA-1004 00193070 CF3950J AmaxaNFreag PC 10RCTHumanDendriticCellKit VAPD-1004 00193084 CF3950J AmaxaNFreag PC 10RCTMEF1Kit VAPG-1004 00193092 CF3950J AmaxaNFreag PC 10RCTMouseNeuralStemCellKit VAPG-1005 00193093 CF3950J AmaxaNFreag PC 10RCTRatNeuralStemCellKit VAPI-1003 00193102 CF3950J AmaxaNFreag PC 10RCTBasicPrimaryNeuronsKit VAPI-1004 00193103 CF3950J AmaxaNFreag PC 10RCTBasicSMCKit VVPA-1006 00192998 CF3950K AmaxaNFreag KT 100RCTMouseTCellKit VAPD-1001 00193081 CF3950J AmaxaNFreag PC 10RCTNHDFKit VAPI-1002 00193101 CF3950J AmaxaNFreag PC 10RCTBasicFibroblastsKit VAPA-1002 00193068 CF3950J AmaxaNFreag PC 10RCTHumanTCellKit VAPH-1001 00193097 CF3950J AmaxaNFreag PC 10RCTMouseESCellKit VAPI-1001 00193100 CF3950J AmaxaNFreag PC 10RCTBasicEndothelialCellsKit VAPA-1003 00193069 CF3950J AmaxaNFreag PC 10RCTHumanCD34CellKit VAPG-1001 00193089 CF3950J AmaxaNFreag PC 10RCTMouseNeuronKit VAPG-1003 00193091 CF3950J AmaxaNFreag PC 10RCTRatNeuronKit VAPI-1005 00193104 CF3950J AmaxaNFreag PC 10RCTBasicEpithelialCellsKit VVPB-1001 00193022 CF3950K AmaxaNFreag KT 100RCTHCAECKit VVPB-1003 00193024 CF3950K AmaxaNFreag KT 100RCTHMVEC-LKit VVPB-1492 00193025 CF3950K AmaxaNFreag KT 100RCTHUVECOLDKit VCO-1001N 00191670 CF3950B AmaxaNFreag PC CellLineOptimizationNucleofector®Kit VS2-00500P 00201525 CF3950T AmaxaNFreag PC Nucleofector™PLUS-2Supplement,500µl VACA-1005 00193064 CF3950H AmaxaNFreag PC 10RCTCellLineKitL VVPA-1010 00192980 CF3950K AmaxaNFreag KT 100RCTMouseBCellKit VVPH-1001 00193045 CF3950K AmaxaNFreag KT 100RCTMouseESCellKit VACA-1001 00193060 CF3950H AmaxaNFreag PC 10RCTCellLineKitR VACA-1002 00193061 CF3950H AmaxaNFreag PC 10RCTCellLineKitT VS1-00150P 00201522 CF3950T AmaxaNFreag PC Nucleofector™PLUS-1Supplement,150µl VPH-5002 00191804 CF3950D AmaxaNFreag PC HumanStemCellNucleofector®StarterKit VPD-1006 00191791 CF3950D AmaxaNFreag PC MEFStarterNucleofector®Kit VPA-1006 00191775 CF3950D AmaxaNFreag PC MouseTCellNucleofector®Kit VVPK-1002 00193054 CF3950K AmaxaNFreag KT 100RCTHMECKit VVPA-1008 00193000 CF3950K AmaxaNFreag KT 100RCTHumanMacrophageKit VVPA-1009 00193001 CF3950K AmaxaNFreag KT 100RCTMouseMacrophageKit VACA-1003 00193062 CF3950H AmaxaNFreag PC 10RCTCellLineKitV VVMI-1021 00192992 CF3950K AmaxaNFreag KT 100RCTBasicParasiteKit2 VVPH-5012 00193046 CF3950K AmaxaNFreag KT 100RCTHumanStemCellKit1 VVPH-5022 00193047 CF3950K AmaxaNFreag KT 100RCTHumanStemCellKit2 VVPA-1005 00192997 CF3950K AmaxaNFreag KT 100RCTHumanNKCellKit VVPF-1001 00193035 CF3950K AmaxaNFreag KT 100RCTHumanChondrocyteKit VPB-1001 00191781 CF3950D AmaxaNFreag PC HCAECNucleofector®Kit VPB-1003 00191783 CF3950D AmaxaNFreag PC HMVEC-LNucleofector®Kit VVPE-1002 00193034 CF3950K AmaxaNFreag KT 100RCTRatCardiomyocyteNeoKit VVPG-1002 00193037 CF3950K AmaxaNFreag KT 100RCTChickenNeuronKit VVPB-1002 00193023 CF3950K AmaxaNFreag KT 100RCTHUVECKit VVPC-1001 00193026 CF3950K AmaxaNFreag KT 100RCTHumanAoSMCKit VVPD-1003 00193030 CF3950K AmaxaNFreag KT 100RCTNHEM-NeoKit VVPL-1004 00198772 CF3950K AmaxaNFreag KT 100RCTMouse/RatHepatocyteKit VPB-1492 00191784 CF3950D AmaxaNFreag PC HUVECOLDNucleofector®Kit VVPI-1003 00193050 CF3950K AmaxaNFreag KT 100RCTBasicPrimaryNeuronsKit VVPA-1011 00193021 CF3950K AmaxaNFreag KT 100RCTMouseDendriticCellKit VVCA-1004 00192948 CF3950I AmaxaNFreag KT 100RCTCellLineKitC VVSI-1003 00193059 CF3950K AmaxaNFreag KT 100RCTBasicNeuronSCNKit VVPA-1001 00192993 CF3950K AmaxaNFreag KT 100RCTHumanBCellKit VVPA-1003 00192995 CF3950K AmaxaNFreag KT 100RCTHumanCD34CellKit VVPA-1004 00192996 CF3950K AmaxaNFreag KT 100RCTHumanDendriticCellKit VVPA-1007 00192999 CF3950K AmaxaNFreag KT 100RCTHumanMonocyteKit VVPD-1002 00193029 CF3950K AmaxaNFreag KT 100RCTHumanKeratinocyteKit VVPD-1004 00193031 CF3950K AmaxaNFreag KT 100RCTMEF1Kit VVPD-1005 00193032 CF3950K AmaxaNFreag KT 100RCTMEF2Kit VVPE-1001 00193033 CF3950K AmaxaNFreag KT 100RCTHumanMSCKit VVPG-1005 00193041 CF3950K AmaxaNFreag KT 100RCTRatNeuralStemCellKit VVPI-1001 00193048 CF3950K AmaxaNFreag KT 100RCTBasicEndothelialCellsKit VVPI-1005 00193052 CF3950K AmaxaNFreag KT 100RCTBasicEpithelialCellsKit VVPI-1006 00198771 CF3950K AmaxaNFreag KT 100RCTBasicGlialCellsKit VVMI-1011 00192950 CF3950K AmaxaNFreag KT 100RCTBasicParasiteKit1 VVPI-1004 00193051 CF3950K AmaxaNFreag KT 100RCTBasicSMCKit VIPI-1003 00194547 CF3952K AmaxaNFreag PC BasicNeuron96-wellADKit VVPI-1002 00193049 CF3950K AmaxaNFreag KT 100RCTBasicFibroblastsKit VVPG-1004 00193039 CF3950K AmaxaNFreag KT 100RCTMouseNeuralStemCellKit VVPG-1001 00193036 CF3950K AmaxaNFreag KT 100RCTMouseNeuronKit V4XP-5032 00197192 CF3950R AmaxaNFreag KT P5PrimaryCell4DXKitS(32RCT) VPA-1010 00191779 CF3950D AmaxaNFreag PC MouseBCellNucleofector®Kit VVPD-1001 00193027 CF3950K AmaxaNFreag KT 100RCTNHDFKit VVCA-1001 00192945 CF3950I AmaxaNFreag KT 100RCTCellLineKitR VVCA-1005 00192949 CF3950I AmaxaNFreag KT 100RCTCellLineKitL VVCA-1002 00192946 CF3950I AmaxaNFreag KT 100RCTCellLineKitT VVPA-1002 00192994 CF3950K AmaxaNFreag KT 100RCTHumanTCellKit VVPG-1003 00193038 CF3950K AmaxaNFreag KT 100RCTRatNeuronKit VVCA-1003 00192947 CF3950I AmaxaNFreag KT 100RCTCellLineKitV VPK-1002 00191813 CF3950D AmaxaNFreag PC HMECNucleofector®Kit VPA-1008 00191777 CF3950D AmaxaNFreag PC HumanMacrophageNucleofector®Kit VPA-1009 00191778 CF3950D AmaxaNFreag PC MouseMacrophageNucleofector®Kit VMI-1021 00191769 CF3950D AmaxaNFreag PC BasicParasiteNucleofector® Kit2 VPH-5012 00191805 CF3950D AmaxaNFreag PC HumanStemCellNucleofector®Kit1 VPH-5022 00191806 CF3950D AmaxaNFreag PC HumanStemCellNucleofector®Kit2 VPA-1005 00191774 CF3950D AmaxaNFreag PC HumanNKCellNucleofector®Kit VPF-1001 00191794 CF3950D AmaxaNFreag PC HumanChondrocyteNucleofector®Kit V4XP-2032 00197189 CF3950R AmaxaNFreag KT P2PrimaryCell4DXKitS(32RCT) VPE-1002 00191793 CF3950D AmaxaNFreag PC RatCardiomyocyteNeoNucleofector®Kit VPG-1002 00191796 CF3950D AmaxaNFreag PC ChickenNeuronNucleofector®Kit VPB-1002 00191782 CF3950D AmaxaNFreag PC HUVECNucleofector®Kit V4XP-4032 00197191 CF3950R AmaxaNFreag KT P4PrimaryCell4DXKitS(32RCT) V4SP-2096 00198753 CF3952D AmaxaNFreag KT P2PrimaryCell96-wellKit(96RCT) VPC-1001 00191785 CF3950D AmaxaNFreag PC HumanAoSMCNucleofector®Kit VPD-1003 00191788 CF3950D AmaxaNFreag PC NHEM-NeoNucleofector®Kit VPL-1004 00198770 CF3950D AmaxaNFreag PC Mouse/RatHepatocyteNucleofector™Kit VPI-1003 00191809 CF3950D AmaxaNFreag PC BasicPrimaryNeuronsNucleofector®Kit VPA-1011 00191780 CF3950D AmaxaNFreag PC MouseDendriticCellNucleofector®Kit VCA-1004 00191668 CF3950B AmaxaNFreag PC CellLineNucleofector®KitC VSPI-1003 00191818 CF3950D AmaxaNFreag PC BasicNeuronSCNNucleofector®Kit VPA-1001 00191770 CF3950D AmaxaNFreag PC HumanBCellNucleofector®Kit VPA-1003 00191772 CF3950D AmaxaNFreag PC HumanCD34CellNucleofector®Kit VPA-1004 00191773 CF3950D AmaxaNFreag PC HumanDendriticCellNucleofector®Kit VPA-1007 00191776 CF3950D AmaxaNFreag PC HumanMonocyteNucleofector®Kit VPD-1002 00191787 CF3950D AmaxaNFreag PC HumanKeratinocyteNucleofector®Kit VPD-1004 00191789 CF3950D AmaxaNFreag PC MEF1Nucleofector®Kit VPD-1005 00191790 CF3950D AmaxaNFreag PC MEF2Nucleofector®Kit VPE-1001 00191792 CF3950D AmaxaNFreag PC HumanMSCNucleofector®Kit VPG-1005 00191799 CF3950D AmaxaNFreag PC RatNeuralStemCellNucleofector®Kit VPH-1001 00191803 CF3950D AmaxaNFreag PC MouseESCellNucleofector®Kit VPI-1001 00191807 CF3950D AmaxaNFreag PC BasicEndothel.CellsNucleofector®Kit VPI-1005 00191811 CF3950D AmaxaNFreag PC BasicEpithelialCellsNucleofector®Kit VPI-1006 00198768 CF3950D AmaxaNFreag PC BasicGlialCellsNucleofector™Kit V4XP-1032 00197188 CF3950R AmaxaNFreag KT P1PrimaryCell4DXKitS(32RCT) VMI-1011 00191768 CF3950D AmaxaNFreag PC BasicParasiteNucleofector® Kit1 VPI-1004 00191810 CF3950D AmaxaNFreag PC BasicSMCNucleofector®Kit V4SP-5096 00198756 CF3952D AmaxaNFreag KT P5PrimaryCell96-wellKit(96RCT) V4XC-3032 00197175 CF3950N AmaxaNFreag KT SGCellLine4DXKitS(32RCT) V4XC-1032 00197173 CF3950N AmaxaNFreag KT SECellLine4DXKitS(32RCT) V4SP-4096 00198755 CF3952D AmaxaNFreag KT P4PrimaryCell96-wellKit(96RCT) V4SP-1096 00198752 CF3952D AmaxaNFreag KT P1PrimaryCell96-wellKit(96RCT) VPI-1002 00191808 CF3950D AmaxaNFreag PC BasicFibroblastsNucleofector®Kit VPG-1004 00191798 CF3950D AmaxaNFreag PC MouseNeuralStemCellNucleofector®Kit VPG-1001 00191795 CF3950D AmaxaNFreag PC MouseNeuronNucleofector®Kit VPD-1001 00191786 CF3950D AmaxaNFreag PC NHDFNucleofector®Kit VCA-1001 00191665 CF3950B AmaxaNFreag PC CellLineNucleofector®KitR VCA-1005 00191669 CF3950B AmaxaNFreag PC CellLineNucleofector®KitL VCA-1002 00191666 CF3950B AmaxaNFreag PC CellLineNucleofector®KitT VPA-1002 00191771 CF3950D AmaxaNFreag PC HumanTCellNucleofector®Kit VPG-1003 00191797 CF3950D AmaxaNFreag PC RatNeuronNucleofector®Kit V4XP-9096 00197193 CF3950R AmaxaNFreag PC PrimaryCellOptimiz.4DXKit(96RCT) V4SC-3096 00198751 CF3952B AmaxaNFreag KT SGCellLine96-wellKit(96RCT) V4SC-1096 00198740 CF3952B AmaxaNFreag KT SECellLine96-wellKit(96RCT) V4XC-2032 00197174 CF3950N AmaxaNFreag KT SFCellLine4DXKitS(32RCT) VCA-1003 00191667 CF3950B AmaxaNFreag PC CellLineNucleofector®KitV V4SP-3096 00198754 CF3952D AmaxaNFreag KT P3PrimaryCell96-wellKit(96RCT) V4SC-2096 00198709 CF3952B AmaxaNFreag KT SFCellLine96-wellKit(96RCT) V4XC-9064 00197176 CF3950N AmaxaNFreag PC CellLineOptimization4DXKit(64RCT) V4SC-9096 00191700 CF3952B AmaxaNFreag PC CellLineOptimiz.96-wellKit(96RCT) VS1-00500P 00201524 CF3950T AmaxaNFreag PC Nucleofector™PLUS-1Supplement,500µl VGA-1003 00191684 CF3950F AmaxaNFreag PC cGMPCellLineNucleofector®KitV VGA-1005 00191686 CF3950F AmaxaNFreag PC cGMPCellLineNucleofector®KitL VSOP-2096 00200021 CF3952I AmaxaNFreag KT P2PrimaryCellsolution(1x96) VSOP-5096 00200024 CF3952I AmaxaNFreag KT P5PrimaryCellsolution(1x96) VSOP-4096 00200023 CF3952I AmaxaNFreag KT P4PrimaryCellsolution(1x96) VSOP-1096 00200020 CF3952I AmaxaNFreag KT P1PrimaryCellsolution(1x96) VSOC-3096 00200019 CF3952I AmaxaNFreag KT SGCellLineSolution(1x96) VSOC-1096 00200016 CF3952I AmaxaNFreag KT SECellLineSolution(1x96) VSOP-3096 00200022 CF3952I AmaxaNFreag KT P3PrimaryCellsolution(1x96) VSOC-2096 00200018 CF3952I AmaxaNFreag KT SFCellLineSolution(1x96) V4XCG-2024 00202415 CF3950F AmaxaNFreag PC cGMPSolutionSF4D-Nucleofector™Kit V4XPG-3024 00202416 CF3950F AmaxaNFreag PC cGMPSolutionP34D-Nucleofector™Kit VZB-1001 00200833 CF3950Q Amaxaotherreag PC MouseTCellNucleofector™medium100ml VASI-1003 00193110 CF3950J AmaxaNFreag PC 10RCTBasicNeuronSCNKit AAL-1001 00194560 CF39581 CellAnalysisInstruments PC Lucetta™Luminometer AWL-1002 00909558 CF39582 CellAnalysisInstruments PC Lucetta™Guarantee,2yearextension AALG-1001 00203777 CF39581 CellAnalysisInstruments PC Lucetta™Luminometer,useddevice VDC-1040 00191671 CF39556 Amaxaotherreag PC pmaxCloning™