Lonza/Keratinocytes (Normal & Diseased)/CC-5034/1 Ea

NormaladultkeratinocytesinKGM-Gold™BulletKit™seenunderlightmicroscopy

ProductOverview:

MostofLonza"sprimarycellsarealsoavailableas proliferatingcells ineitherflasksorplates,oraspelletsinRNALater^®.

DiseasedHumanEpidermalKeratinocytes(D-HEK) areisolatedfromhumandonorsthathavebeendiagnosedwithdiabetestypeIIdisease.Furtherdonorinformation,includingmedications,BMI,anddiseasedurationmaybeobtainedbycontactingLonzaScientificSupport.  DiseasedcellsaregrowninthesameoptimizedmediasystemasNormalHumanKeratinocytes.

SelectReferenceswithLonza’sKeratinocytes:

1)Protectivebarrier:Keratinocytes werestimulatedwithhistamineorhistaminereceptorligandstoshowtheformationofadefectiveskinbarrier. GschwandtnerM,MildnerM, MlitzV, GruberF, EckhartL,WerfelT,GutzmerR,EliasPMandTschachlerE. Histaminesuppressesepidermalkeratinocytedifferentiationandimpairsskinbarrierfunctioninahumanskinmodel.EuropeanJournalofAllergyandClinicalimmunology(2013);68(1):37–47. 

2)Skinmodel:Neonatalkeratinocyteswereculturedinamicrofluidicsystemtopreciselyexaminethemorphology,behaviorandviABIlityofindividualcells. AdrianT.O’Neill,NancyA.Monteiro-Riviere,andGlennM.Walker.Characterizationofmicrofluidichumanepidermalkeratinocyteculture.Cytotechnology(2008);56(3):197–207. 

3)Cancer:KeratinocyteswereusedinthisstudytodemonstratethatdysregulatedactivationoftypeIinterferoninnateimmunityisimplicatedinthemolecularprocessestriggeredbyanti-EGFRdrugsandleADIngtopersistentskininflammation. CarrieHS,HongY,YunboL,JenniferSM,SrideviB,GayleneS,JudithACandThomasRS. EGFreceptorsignalingblocksarylhydrocarbonreceptor-mediatedtranscriptionandcelldifferentiationinhumanepidermalkeratinocytes.ProceedingsofNationalAcademyofSciences(2009);106(11):4266-71. 

4)HumanPapillomaviruses(HPV)mediatedskincancerresponse:Aninfectionoftheskinwithhumanpapillomaviruses(HPV)mayresultinbenigntumorswithlimitedgrowth,whichtendtoregressspontaneouslyandcausesnon-melanomaskincancerdevelopmentinepidermodysplasiaverruciformis(EV)andimmunosuppressedpatients. ThepresentstudyutilizedpooledkeratinocytesculturedinKGM^TM-GoldMediumfromseveralhealthydonors.ThedatashowedthatbetaPVmayincreasethenumberofstemcell-likecellspresentduringearlycarcinogenesisandthusenablethepersistenceandaccumulationofDNAdamagenecessarytogeneratemalignantstemcells. MartinH,AdrianB,CinziaB,MarisaG,JohnD,AlanS,HerbertP,IanMandBakiA.Expressionofbetapapillomavirusoncogenesincreasesthenumberofkeratinocyteswithstemcell-likeproperties.JournalofVirology(2013);87(22):12158-12165. 

New!PurchaseourAdultKeratinocyteCellCultureKit(catalogno.192627B)andgetadiscountedpriceoncellsandmedia.Thekitconsistsofacryopreservedampuleofadultkeratinocytes(catalogno.192627)andKGM™-GoldBulletKit™GrowthMedia(catalogno.192060)