1 mg of BHK21 Whole Cell Lysate(Catalog #8904),Altogen,,Altogen,+,,Array.Array.Array蚂蚁淘厂家直采.

Altogen/Whole Cell Lysate/1 mg of BHK-21 Whole Cell Lysate(Catalog #8904)
Manufacturer of 120+ Cell Transfection Reagents and Tissue Targeted Delivery Kits Altogen Biosystems is a biotechnology company that develops and manufactures transfection kits for life sciences research, drug discovery and development. Altogen ...
Description

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WholeCellLysate,1mg(2mg/ml)

GeneralNotes:
Growthmedia:DMEMsupplementedwith10%FBS

CelllysatewaspreparedbyhomogenizationinRIPAbuffer(25mMTrisHClpH7.6,150mMNaCl,1%NP-40,1%sodiumdeoxycholate,0.1%SDS)supplementedwith5µg/mlofaprotininand5µg/mlofleupeptin.Celldebriswereremovedbycentrifugation(5minutesat12,000RPM).ConcentrationoftotalproteinwasdeterminedusingBCAproteinassay.

StorageInstructions
Shippedat4°C.Upondeliveryaliquot.Storeat-80°C.Avoidfreeze/thawcycle.

Volume
0.5ml

Concentration
1mgat2mg/ml

Purity
WholeCellLysate

AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

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Altogen Biosystems is a biotechnology company that develops and manufactures transfection kits for life sciences research, drug discovery and development. Altogen In Vivo transfection reagents provide effective delivery of biomolecules into target tissues. Cell transfection kits are optimized for specific cancer cell lines and primary cell cultures. Efficient delivery of cargo molecules (DNA, RNA, proteins) enabled by advanced reagent formulation and optimized transfection protocols. Altogen Biosystems develops new in vitro and in vivo delivery technologies by applying polymer chemistry, molecular and cell biology expertise. Altogen Biosystems offers preoptimized transfection kits and electroporation products for more than 120 cancer cell lines and primary cell types, and in vivo transfection reagents for tissue-targeted delivery.Altogen’s transfection reagents enable the delivery of cargo molecules, including plasmid DNA, various types of RNA (mRNA, siRNA, shRNA, microRNA), proteins, and small molecules both in vitro (cancer cell lines) and in vivo (tissue targeted reagents for animal research). Transfection is the delivery of exogenous molecules into cultured cells and is commonly used to study gene function, modulation of gene expression, biochemical mapping, and protein production. Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells due to cellular cytotoxicity and transfection efficiencies vary dramatically and depend on the reagent, protocol, and cell type used. Altogen Labs provides GLP-compliant laboratory contract research services. Our biology CRO services include xenograft efficacy, pharm/tox studies and safety tests for IND applications, assay development (ELISA, IC50, qPCR), over 80 xenograft animal models, RNAi and gene silencing services. Altogen’s cell culture scientists transform cell lines of choice to stably express gene of interest via development of stable cell line in just 28 days.

苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司，以常规生物学实验外包与生物学试剂为基础，放眼于生命科学领域的前沿技术服务，公司技术骨干有着多年的生物学科研背景，毕业于国内外名牌大学，并且有着丰富的项目经验，了解国内外科研行情，我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点，和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括：抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人，认真做事，服务至上“，希望用我们的专业知识和项目经验为国内科研客户解决科研问题，祝您科研之路畅通无阻！

脂质体或许大家更熟悉，它的历史已超过20年，是最受欢迎的体外导入载体。脂质体结合或包围核酸，进而被细胞吸收。Invitrogen公司的脂质体Lipofectin、Lipofectamine、Lipofectamine 2000已畅销十余年。脂质体还是携带小分子RNA进入细胞的有力武器。Ambion（现属于生命科技公司）就开发出siRNA转染试剂-siPORT NeoFX，Invitrogen也修改了阳离子脂质体的配方，专门为小分子RNA推出RNAiMAX。
当然，转染不仅仅限于细胞系，许多研究人员将他们的目光投向体内转染。一些生物公司如Polyplus-transfection、Altogen Biosystems等利用脂质体或可生物降解的聚合物作为载体，将DNA片段或siRNA导入体内。然而，它们都不是理想的载体。脂质体的导入效果虽比聚合物要持久，但它可能引起更多的免疫反应，这也不是研究人员所期望的。也正是这些不足，导致了近几年纳米颗粒的出现。2007年，Sigma-Aldrich推出了N-TER纳米颗粒siRNA转染试剂，让siRNA结合在纳米颗粒的表面，通过N-TER肽段导入体内。
在RNAi研究领域，越来越多的研究人员开始对高通量的筛选感兴趣。瑞典Cellectricon公司也注意到这一点，他们认识到转染是RNAi筛选中的主要瓶颈之一，急需一种高通量的转染方法。于是他们花了两年的时间，开发出第一台全自动的转染系统-Cellaxess HT电穿孔仪。这台仪器能够自动移液，自动在细胞中加入siRNA，自动将移液吸头转换成电穿孔元件，并进行电穿孔，最后加入细胞培养基进行复苏。Cellaxess HT每天能转染50000个孔。蚂蚁淘之前介绍过的Nucleofector核转染系统也是类似的电穿孔仪器，它能够对96孔板进行转染。
说到底，转染的目标其实很简单，就是用最少量的DNA或RNA去转染最多的细胞。当然，还要尽量减少或避免对细胞的毒性、脱靶效应和免疫反应。别看这个目标简单，实现起来还真不容易。无论是试剂还是仪器，都有需要改进的地方，同时，新技术也在不断萌芽。也许在不久的将来，转染将不再是实验中的瓶颈，轻轻松松就能实现。