Altogen/ASMC Transfection Kit (Aortic Smooth Muscle Cells)/1.5 ml CRISPR(Catalog #2108)

Description

ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).

Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).

TransfectionReagentforASMCCells(AorticSmoothMuscleCells)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroASMC transfectionprotocol:[PDF]
• Download ASMCCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforASMCcellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsASMCTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

ASMC CellLine:

AccordingtoAmericanHeartAssociation,nearly92.1millionAmericanssufferfromacardiovasculardisease(CVD)ofsomesort.PreclinicalresearchiscriticalinunderstandinghowthearteriesrespondtovariousBIOLOGicalandchemicalfactors,andhencedeviseinnovativemethodstoaddressCVDs.HumanAorticSmoothMuscleCellsarederivedfromtunicaintimaandtunicamediaofplaque-freeaortatissue.Thetunicaintimaistheinnerlayerofthearterycomprisedofalining,anetworkofconnectivetissue,andalayerofelasticfibers;thetunicamedia,ormiddlecoat,iscomposedmostlyofsmoothmusclecellsandelasticfibersarrangedinspirallayers.ArterialSmoothMuscleCell(ASMC)linehasbeenusefulinthestudyofatherosclerosis,avasculardisordercharacterizedbylossofflexibilityinbloodvesselsduetotheaccumulationofdeadwhitebloodcells,cholesterol,andcrystallizedsalts,whichleadstonarrowingofarteriesandmaycausefurtherproblemssucharrhythmiaandstrokes.Humanarterialsmoothmusclecellscansynthesizecollagen,elastin,myosin,andglycosaminoglycan.ASMCcellsrespondtovariousfactorsbyproliferatingordifferentiating.Thesecellsareusedtostudyofhumanvasculardisorders,includingangiogenesis,stroke,vascularbiologyandalsodiabetes.HumanaorticsmoothmusclecellsareinsulinresistantatthereceptorlevelbutsensitivetoIGF1andIGF2.CurcuminpreventsthemigrationofhumanaorticsmoothmusclecellsbyinhibitingofMMP-9expression.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheASMCcellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofGAPDHinASMCcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoASMCcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:

AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

SelectedASMC transfectionreagentcitationreferences:

• FEBSJ.2016.283:1767–1781.TransforminggrowthfactorB1antagonizesthetranscription,expressionandvascularsignalingofguanylylcyclase…Senetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpreclinicalresearch,INDapplications,anddrugdevelopment.OurbiologyCROservices include:Xenograftservices(over60xenograftmodels),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1707)
• 1.5ml(Catalog#1708)
• 1.5mlCRISPR(Catalog#2108)
• 8.0ml(Catalog#7022)