TISSUE CULTURE STOCK SOLUTIONS AND MEDIA

MSMEDIUMFORARABIDOPSIS

To 990 ml H2Oadd:Sucrose...........10.0gMOPS..............0.5gAgar..............8.0gAdjustpHto5.7with1MKOH.Autoclave.Whencooledaddsterilestocksolutionsof:KNO3...............20.0ml940mM(95.00g/L)NH4NO3.............20.0ml1000mM(80.04g/L)KH2PO4.............12.5ml100mM(13.60g/L)MgSO47H2O..........15.0ml100mM(24.65g/L)CaCl27H2O..........10.0ml300mM(44.00g/L)Glycine............2.67ml10mMNicotinate.........0.4ml10mMThiamin............0.02ml10mMPyridoxineHCl......0.24ml10mMMyoinositol........5.5ml100mMFeSO47H2O.........1.0ml(2.780g/100ml)Na2EDTA...........1.0ml100mM(3.720g/100ml)MnSO44H2O..........1.0ml100mM(1.690g/100ml)ZnSO44H2O..........3.0ml10mM(0.288g/100ml)H3BO3.............1.0ml100mM(0.618g/100ml)KI.................0.5ml10mM(0.166g/100ml)CuSO45H2O.........0.01ml10mM(0.170g/100ml)Na2MoO42H2O.......0.1ml10mM(0.242g/100ml)CoCl26H2O.........0.01ml10mM(0.238g/100ml)
B5MEDIUMFORARABIDOPSIS
To990mlH2Oadd:Sucrose...........20.0gMOPS..............0.5gAgar..............8.0gAdjustpHto5.7with1MKOH.Autoclave.Whencooledaddsterilestocksolutionsof:KNO3...............26.3ml940mM(95.00g/L)NaH2PO4H2O.........11.0ml100mMMgSO47H2O..........10.0ml100mM(24.65g/L)(NH4)2SO3..........10.0ml100mMCaCl27H2O..........3.3ml300mM(44.00g/L)Myoinositol........5.5ml100mMThiamine...........3.0ml10mMNicotinate.........0.8ml10mMPyridoxineHCl......0.48ml10mM10XMicronutrients.10.0ml(seebelow)Sequestrene330Fe.10.0ml(0.28g/100ml)ForCallusInducingMedium(CIM)add:2,4-D.....................0.5mlKinetin...................0.05mlForShootInducingMedium(SIM)add:IAA.......................0.15ml6-dimethylaminopurine.....5.0ml
10XMicronutrients(per100ml)MnSO44H2O..........0.6ml100mM(1.690g/100ml)ZnSO44H2O..........10.07ml10mM(0.288g/100ml)H3BO3.............4.9ml100mM(0.618g/100ml)KI.................4.5ml10mM(0.166g/100ml)CuSO45H2O.........2.3ml10mM(0.170g/100ml)Na2MoO42H2O.......1.0ml10mM(0.242g/100ml)CoCl26H2O.........1.0ml10mM(0.238g/100ml)