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Gendepot/His-Tag Mouse Monoclonal Antibody, 1mg/ml, Ab1005/1mg/Ab1005-101188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Gendepot/His-Tag Mouse Monoclonal Antibody, 1mg/ml, Ab1005/1mg/Ab1005-101

ReactivityHuman
ClonalityMonoclonal , HIS.H8
Host Mouse
IsotypeIgG2b
Form Liquid, 1mg/ml in 10mM PBS, pH 7.2 with 0.05% NaN3
ApplicationWB - 1:1000 ~ 1:3000
IP - 1:50 ~ 1:200
IF/ICC - 1:200 ~ 1:500
Epitope tags provide a method to localize gene products
in a variety of cell types, study the topology of proteins
and protein complexes, identify associated proteins, and
characterize newly identified, low abundance or poorly
immunogenic proteins when protein specific antibodies
are not available. Expression of recombinant proteins
in E-coli as a fusion protein with neighboring histidine
residues is one of the most popular methods of epitope
tags.
The affinity of the histidine-tag motif to Ni2+ by chelation
is strong and selective enough to enable purification of
the protein tohomogeneity by affinity chromatography
on a Ni2+ -NTA adsorbent.
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