常用的βactin 引物序列

humanactinfctccatcctggcctcgctgthumanactinrgctgtcaccttcaccgttccproductsize:268rabbitactinragtgcgacgtggacatccgrabbitactinftggctctaacagtccgcctagproductsize:295mouseactinrcgttgacatccgtaaagaccmouseactinfaacagtccgcctagaagcacproductsize:281ratactinfTCAGGTCATCACTATCGGCAATratactinrAAAGAAAGGGTGTAAAACGCAproductsize:432humanactinrgagctacgagctgcctgacghumanactinfcctagaagcatttgcggtggproductsize:416mouseactinftcatcactattggcaacgagcmouseactinraacagtccgcctagaagcacproductsize:399ratactinfCCCATCTATGAGGGTTACGCratactinrTTTAATGTCACGCACGATTTCproductsize:150rabbitactinftcttccagccctccttcctgrabbitactinrcgtttctgcgccgttaggtproductsize:409内参基因名称引物引物最佳退火扩增基因库序列号引物名称序列位置Tm温度C长度HumanactinbetaF305ctgggacgacatggagaaaa305-32452.3BC002409R868aaggaaggctggaagagtgc868-84952.659.4564F1379agcgagcATCCcccaaagtt1379-139857.3R1663gggcacgaaggctcatcatt1663-164456.354285RatactinbetaF18cacccgcgagtacaaccttc18-3754.5NM_031144R224cccatacccaccatcacacc224-20554.460.4207F694gagagggaaatcgtgcgtgac694-71454R1146catctgctggaaggtggaca1146-112753.257.1452MouseactinbetaF91atatcgctgcgctggtcgtc91-11057.5NM_007393R607aggatggcgtgagggagagc607-58857.860.4517F1566gtccctcaccctcccaaaag1566-158554.5F1831gctgcctcaacacctcaaccc1831-181154.455.7266humanGAPDHF369agaaggctggggctcatttg369-38855.6BC004109R626aggggccatccacagtcttc626-60755.157.5258F66aggtcggagtcaacggatttg66-8652.5R597gtgatggcatggactgtggt597-57852.657532RatGAPDHF50cagtgccagcctcgtctcat50-6955BC059110R644aggggccatccacagtcttc644-62555.158.3595F1029acagcaacagggtggtggac1029-104854.4R1280tttgagggtgcagcgaactt1280-126154.857.7252MouseGAPDHF306aggccggtgctgagtatgtc306-32554M32599R835tgcctgcttcaccaccttct835-81654.658.7530F49ggtgaaggtcggtgtgaacg49-6855.1R281ctcgctcctggaagatggtg281-26254.556.8233Rat18SrRNAF1865tgcggaaggatcattaacgga1865-188658.6M11188R2164agtaggagaggagcgagcgacc2164-21435959.3300human18SrRNAF1565cagccacccgagattgagca1565-158458.3M10098R1816tagtagcgacgggcggtgtg1816-179758.359253mouse18SrRNAF56aggggagagcgggtaagaga56-7554.4K013364R296ggacaggactaggcggaaca296-2775462241