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| 货号： | 暂无 |
| 数量： | 大量 |
| 供应商： | eBioscience |
| 保存条件： | 4℃ |
| 保质期： | 1年 |
| 小鼠细胞因子:细胞内快速染色指南 | ||||||
| 小鼠细 胞因子 | 细胞来源 | 激活剂及剂量、时间 | 孵化时间 | 重复刺激 | 胞内阻断剂 | 抗体 |
| GM-CSF | mousespleen | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | MP1-22E9 |
| IFN-γ | mousespleen | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | XMG1.2 |
| IL-1α | mousePEC | mIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | ALF-161 |
| IL-1β | mousePEC | mIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | polyclonal |
| IL-2 | mousespleen | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | JES6-5H4 |
| IL-4 | mousespleen | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | BVD6-24G2, |
| 11B11 | ||||||
| IL-5 NEW! | mousesplenicCD4 | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | TRFK5 |
| IL-6 | mousePEC | mIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | MP5-20F3 |
| IL-10 | mousespleen | ConA(3ug/ml)(2d)/IL-2(20ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | anti-CD3(10ug/mlimmobilized)+anti-CD28(2ug/mlsoluble)5hr | BrefeldinA | JES5-16E3, |
| JES5-2A5 | ||||||
| IL-12/IL-23(p40) | mousePEC | mIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | C17.8 |
| IL-17ANEW! | CD4+CD25-Tcellsfrommousespleenplusday10bonemarrow-deriveddendriticcells | anti-CD3,anti-IL-4,anti-IFN-γ,recTGF-β,recIL-6 | 4d | PMA/Iono5hr | Monensin | eBio17B7 |
| IL-17FNEW! | mouseCD4 | IL-17induction | 6d | PMA/Iono5hr | Monensin | eBio18F10 |
| MCP-1/CCL2NEW! | mousePEC | LPS100ng/ml | 24hr | - | BrefeldinA | 2H5 |
| TNF-α | mousePEC | mIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | MP6-XT22, |
| TN3-19 | ||||||
| Annotations:mousePEC=mousethioglycolate-elicitedperitonealmacrophages;ConA=ConcanavalinA;Iono=Ionomycin;LPS=Lipopolysaccharide;PMA=PhorbolMyristateAcetate;2d=2dayculture;5hr=5hourculture | ||||||
| 人细胞因子:细胞内快速染色指南 | ||||||
| 人细胞 因子 | 细胞来源 | 激活剂及剂量、时间 | 孵化时间 | 重复刺激 | 胞内阻断剂 | 抗体 |
| IFNα2 | PMBC | CpGAODN2216(20ug/ml) | 20hours | - | BrefeldinA(added2-4hrpoststimulation) | 225.C |
| IFN-γ | PBMC | PMA(30-50ng/ml)/Iono(1ug/ml) | 5hr | - | BrefeldinA | 4S.B3 |
| IL-1α | PBMC | LPS100ng/ml | 24hr | - | BrefeldinA | 364/3B3-14 |
| IL-1βNEW! | PBMC | LPS100ng/ml | 24hr | - | BrefeldinA | CRM56 |
| IL-1RA | PBMC | LPS100ng/ml | 24hr | - | BrefeldinA | CRM17 |
| IL-2 | PBMC | PMA(30-50ng/ml)/Iono(1ug/ml) | 5hr | - | BrefeldinA | MQ1-17H12 |
| IL-4 | PBMC | anti-CD3(10µg/ml,immobilized)+anti-CD28(2µg/ml,soluble)+IL-2(10ng/ml)+IL-4(20ng/ml)(2d);IL-2(10ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | PMA(5ng/ml)+Ionomycin(500ng/ml)(4hr) | BrefeldinA | MP4-25D2, |
| 8D4-8 | ||||||
| IL-5 | CD4 | anti-CD3(10µg/ml,immobilized)+anti-CD28(2µg/ml,soluble)+IL-2(10ng/ml)+IL-4(20ng/ml)(2d);IL-2(10ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | PMA(5ng/ml)+Ionomycin(500ng/ml)(4hr) | BrefeldinA | TRFK5, |
| JES1-5A10 | ||||||
| IL-6 | PBMC | LPS100ng/ml | 5hr | - | BrefeldinA | MQ2-13A5 |
| IL-10 | PBMC | Th2polarizingcultures | 6d | PMA(50ng/ml)+ionomycin(1ug/ml) | Monensin | JES3-9D7 |
| IL-12/IL-23(p40) | PBMC | hIFNγ(100ng/ml)(2hr)/LPS(100ng/ml)(22hr) | 2hr/22hr | - | BrefeldinA | C8.6 |
| IL-13NEW! | CD4 | anti-CD3(10µg/ml,immobilized)+anti-CD28(2µg/ml,soluble)+IL-2(10ng/ml)+IL-4(20ng/ml)(2d);IL-2(10ng/ml)+IL-4(20ng/ml)(3d) | 2d/3d | PMA(5ng/ml)+Ionomycin(500ng/ml)(4hr) | BrefeldinA | PVM13-1 |
| IL-17ANEW! | PBMC | PMA(30-50ng/ml)/Iono(1ug/ml) | 5hr | Monensin | eBio64CAP17, | |
| eBio64DEC17 | ||||||
| IL-21 | PBMC | PMA(30-50ng/ml)/Iono(1ug/ml) | 4-7hror12-18hr | BrefeldinA | eBio3A3-N2 | |
| MCP-1/CCL2NEW! | PBMC | LPS100ng/ml | 24hr | - | BrefeldinA | 2H5, |
| 5D3-F7 | ||||||
| TNF-α | PBMC | PMA(30-50ng/ml)/Iono(1ug/ml) | 5hr | - | BrefeldinA | MAb11 |
| TNF-β | PBMC | anti-CD3(10µg/ml,immobilized)+IL-2(10ng/ml)(2d);IL-2(10ng/ml)(3d) | 2d/3d | PMA(5ng/ml)+Ionomycin(500ng/ml)(6hr) | BrefeldinA | 359-81-11 |
| Annotations:Iono=Ionomycin;PMA=PhorbolMyristateAcetate;LPS=Lipopolysaccharide;2d=2dayculture;5hr=5hourculture;LPSforactivationofhumanPBMCobtainedfromSigma(#L-8274) | ||||||