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echelon-inc/Autotaxin Inhibitor Screening Kit/1ea/K-4200-1ea


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CatalogNo.: K-4200

Description: Echelon'sAutotaxinInhibitorAssayusespurifiedautotaxin(ATX)andtheautotaxinsubstrateFS-3,whichfluorescesuponcleavage.Theassayisperformedina96wellplateandquantifiestheautotaxinactivityinthepresenceoftheuser'ssamplesbymeasuringchangesinfluorescenceovertime.

ProductBackground:TheAutotaxinInhibitorAssay,developedbyEchelonBiosciencesInc.,usesthefluorogenicautotaxinsubstrateFS-3.FS-3isanLPCanaloguethatisconjugatedwithbothafluorophoreandaquencher.Initsnativestatethequencherinterfereswiththefluorophore’sfluorescence.OnceautotaxincleavesFS-3,thefluorophorebecomesliberatedfromthequencher,resultinginincreasedfluorescence.
Percentautotaxininhibitionisdeterminedbyreferencingnoinhibitorcontrols.Theautotaxininhibitor,BrP-LPA,issuppliedtopositivelycontrolforautotaxininhibition.TheassayisresistanttomodestconcentrationsofDMSOandisperformedinacontinuousassayformat,whichhastheadvantageofbeinglesssusceptIBLetoerrorarisingfrommis-timingtheadditionofcomponentstodifferentsamplewells.

FeaturedinPublications:
1.Altman,M.K.,Gopal,V.,Jia,W.,Yu,S.,Hall,H.,Mills,G.B.,McGinnis,A.C.,Bartlett,M.G.,Jiang,G.,Madan,D.,Prestwich,G.D.,Xu,Y.,Davies,M.A.,&Murph,M.M.,TargetingmelanomagrowthandviABIlityrevealsdualisticfunctionalityofthephosphonothionateanalogueofcarbacyclicphosphatidicacid.2010.MolCancer9,140.
2.Jiang,G.,Madan,D.,&Prestwich,G.D.,AromaticphosphonatesinhibitthelysophospholipaseDactivityofautotaxin.BioorgMedChemLett.

RelatedProducts:
AutotaxinAssaykits:AutotaxinActivityAssay(K-4100),HighThroughputAutotaxinInhibitorScreeningKit(K-4200HTS)
AutotaxinSubstrate;FS-3(L-2000)
AutotaxinInhibitors;BrP-LPA(L-7416),HA130(B-0701),PF8380(B-0702),andThio-ccPA(L-7118)
AutotaxinEnzyme,active(E-4000)

NoticetoPurchaser:EchelonBiosciencesproductsaresoldforresearchanddevelopmentpurposesonlyandarenottobeincorporatedintoproductsforresalewithoutwrittenpermissionfromEchelonBiosciences.ThecompoundFS-3anditsuseinassayingforLysophospholipaseDactivityarecoveredbyEchelonBiosciencesInc.USpatent7,989,663.Thepurchaseofthisproductincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser'sowninternalresearch.

ProductKeywords:ATX,LPA,FS-3,LPC,lysophospholipaseD,BrP-LPA

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