mRNA synthesis In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA) TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8) A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T7标签是一种表位标签,由T7噬菌体基因10的前导序列编码的11肽组成。该基因编码T7主要衣壳蛋白,其功能还不清楚。表位标签可用于标记和检测蛋白,适用于免疫印迹、免疫沉淀和免疫染色技术。由于其体积小,它们不太可能影响标记蛋白的生化特性。
T7标签通常与靶蛋白的N端或C端连接,使标记蛋白可用免疫化学的方法进行分析和可视化。T7标签作为一般的表位标签被广泛用于许多表达载体,包括基于T7 RNA聚合酶表达系统的pET系统[1]。
参考文献:
1.Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.
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