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Oxford Biomedical Research（OBR）公司由Denis M. Callewaert博士于1984年建立。OBR公司开发，生产和销售检测试剂盒，重组蛋白和应用于生物医学和制药研究的抗体，现已具有400多种产品。我们产品的重点是对引发多种疾病的两大风险因素氧化应激和慢性炎症的生物标志物的识别与检测。我们采用专有技术，开发组织和疾病的特异性生物标志物。
检测试剂盒
OBR公司拥有超过25年的开发和生产ELISA检测试剂盒经验，专注于类花生酸代谢、氧化压力、蛋白质组、信号转导、心血管生物学和的ELISA检测。
BACKGROUND
Cortisol is also known as hydrocortisone when it is used medicinally, usually as a supplement or for its anti-inflammatory properties. Knowing plasma cortisol levels is useful in identifying a serious lack of cortisol usually found in Addison's disease, or a serious over production of cortisol as in Cushing's syndrome. Cortisol analysis is also frequently used to evaluate the stress levels of a subject. This ELISA is designed for detecting cortisol in biological fluids including urine, plasma, and saliva.
CORTISOL ASSAY PRINCIPLE
This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of cortisol in biological samples including urine, saliva, and plasma. Briefly, cortisol in the samples or standards competes with cortisol conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for cortisol coated on the microplate. The HRP activity results in brilliant blue color development when the substrate is added, with the intensity of the color development proportional to the amount of cortisol-HRP bound and inversely proportional to the amount of unconjugated cortisol in the samples or standards.
When measuring urine samples, it is recommended to normalize samples to creatinine to account for variations in fluid intake and excretion. Creatinine is excreated at a constant rate and can be used to normalize the rate of excretion of other analytes.
CREATININE ASSAY KIT (CR01)
EA85 Typical Standard Curve