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8-Hydroxy-2’-deoxyguanosine (8-OHdG) is excised during the repair of oxidative damage to dG sites in DNA and has been widely used as a biomarker for oxidative damage. This competitive ELISA kits employs a monoclonal antibody specific for 8-OHdG quantification in tissue, serum, plasma or urine samples. Each kit contains sufficient reagents and standards for the replicate quantification of 8-OhdG in up to 18 samples. Results are read at 450 nm on a microplate reader.
References1. Cutler RG. Am.J.Clin.Nutr. 50:373S-379S, 1991.2 Kasai H et al. Carcinogenesis 7:1849-51, 1986.3. Fraga CG et al. Proc.Natl.Acad. Sci.USA 87:4533-4537, 1990.4. Shigenaga MK, Ames BN. Free Rad Biology and Medicine 10:211-6, 1991.5. Kantha SS et al. Biochem.Biophys. Res.Comm. 223:278-82, 1996.6. S. Toyokuni et al. Osawa. Lab.Invest. 76:365-74, 1997.7. M. Erhola et al. FEBS Letters 40:287-91, 1997.
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