Invitrogen公司A21202抗体现货|赛默飞|thermofisher

Donkeyanti-MouseIgG(H+L)HighlyCross-AdsorbedSecondaryAntibody,AlexaFluor488

Invitrogen公司A11032抗体现货

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ProductDetails

TestedApplications

Dilution

Immunocytochemistry(ICC)

0.2µg/mL

Immunofluorescence(IF)

1:2000

Immunohistochemistry(IHC)

1-10µg/mL

PublishedApplications

Immunocytochemistry(ICC)

See17publicationsbelow

Immunohistochemistry(IHC)

See6publicationsbelow

Immunohistochemistry(Paraffin)(IHC(P))

See3publicationsbelow

Immunohistochemistry(Frozen)(IHC(F))

See5publicationsbelow

FlowCytometry(Flow)

See1publicationbelow

MiscellaneousPubMed(MISC)

See36publicationsbelow

ProductSpecifications

SpeciesReactivity

Mouse

Host/Isotype

Donkey/IgG

Class

Polyclonal

Type

SecondaryAntibody

Immunogen

GammaImmunoglobinsHeavyandLightchains

Conjugate

AlexaFluor®488

Excitation/EmissionProfile

Viewspectra 

Form

Liquid

Concentration

2mg/mL

Purification

purified

Storagebuffer

PBS,pH7.5

Contains

5mMsodiumazide

Storageconditions

4°C,storeindark

RRID

AB_141607

Target

IgG

AntibodyForm

WholeAntibody

ProductSpecificInformation

Tominimizecross-reactivity,thesedonkeyanti-mouseIgGwholeantibodieshavebeenaffinity-purifiedandshowminimumcross-reactivitytobovine,chicken,goat,guineapig,hamster,horse,human,mouse,rat,andsheepserumproteins.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteins frompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperiments(e.g.,flowcytometry)wherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretheremaybethepresenceofendogenousimmunoglobulins.

AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor488dyeisabright,green-fluorescentdyewithexcitationideallysuitedtothe488nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor488dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundancebiologicalstructureswithgreatsensitivity.AlexaFluor488dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.

Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.

Background/TargetInformation

WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.Thebreadthoffluorescent Markersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.

Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.

Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.

ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.