PromoCell 提供 人类软骨细胞 ！

软骨细胞

“人软骨细胞”，Human Chondrocytes (HCH)，500,000 Human Chondrocytes, cryopreserved。
产品的详细信息如下，请点击链接查看：
http://www.promocell.com/fileadmin/promocell/PDF/C-12710.pdf

人类软骨细胞（HCH）是从膝关节和髋关节的正常人类关节软骨分离获得的（可以提供产品批次对应的来源信息）。一般在3D球形体上常规检测 PromoCell HCH 的细胞分化能力。
    关节软骨覆盖骨骼间的关节，因此不含任何血管、淋巴管或神经纤维。关节软骨只由一种特别的细胞类型组成，即软骨细胞，可以产生和维持软骨的细胞外基质，即胶原（大都为 II 型胶原）和蛋白多糖（主要为聚集蛋白多糖）。

 

推荐的培养基及试剂

•   软骨细胞生长培养基         
•   细胞分离试剂盒
•   Cryo-SFM 

 
应用范围

•   骨性关节炎研究
•   关节研究
•   生理学研究
•   软骨细胞分化实验
•   透明软骨研究

 
特点

•   取自髋关节和膝关节
•    通过阿利辛蓝染色HCH球状体来检测细胞分化
•    在第二代时用液氮冻存   

 

相关产品

•   HCH 细胞团
•   PromoFectin 转染试剂

 
Articular cartilage covers the bones in the major joints of the human body, e.g. knee and hip joints. It is composed of only one specialized cell type, Chondrocytes. Chondrocytes produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans. The isolated, differentiated cells express collagen type II; however, during prolonged in vitro cultivation, dedifferentiation begins and collagen type II expression declines.
PromoCell Human Chondrocytes (HCH) are produced at PromoCell’s cell culture facility and are available from knee and hip joint cartilage tissue.
Shortly after isolation, all PromoCell Human Chondrocytes are cryopreserved at passage 2 (P2) using PromoCell’s proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at PromoCell.

 

知识库

To view literature references for this product, please click here.
Shear stress modulates macrophage-induced urokinase plasminogen activator expression in human chondrocytes
Yeh et al.; Arthritis Res Ther. 2013 Apr 18;15(2):R53
Inducible hydrogen sulfide synthesis in chondrocytes and mesenchymal progenitor cells: is h(2) S a novel cytoprotective mediator in the inflamed joint?
Fox et al.; J Cell Mol Med. 2012 Apr;16(4):896-910
Quaternary ammonium-melphalan conjugate for anticancer therapy of chondrosarcoma: in vitro and in vivo preclinical studies
Peyrode et al.; Invest New Drugs. 2012 Aug;30(4):1782-90
Pravastatin suppresses matrix metalloproteinase expression and activity in human articular chondrocytes stimulated by interleukin-1beta
Baker et al.; J Orthop Traumatol. 2012 Sep;13(3):119-23
Recellularization of decellularized human adipose-tissue-derived extracellular matrix sheets with other human cell types
Kim et al.; Cell Tissue Res. 2012 Jun;348(3):559-67
In vitro assessment of human chondrocyte viability after treatment with local anaesthetic, magnesium sulphate or normal saline
Baker et al.; Knee Surg Sports Traumatol Arthrosc. 2011 Jun;19(6):1043-6
Cellular and molecular mechanisms of anti-inflammatory effect of Aflapin: a novel Boswellia serrata extract
Sengupta et al.; Mol Cell Biochem. 2011 Aug;354(1-2):189-97
Characterization of the response of primary cells relevant to dialysis-related amyloidosis to beta2-microglobulin monomer and fibrils
Porter et al.; PLoS One. 2011;6(11):e27353
Comparative efficacy and tolerability of 5-Loxin and Aflapin against osteoarthritis of the knee: A double blind, randomized, Placebo controlled clinical study
Sengupta et al:, Int. J. Med. Sci. 2010, 7: 366-377
Resistance to TRAIL-induced apoptosis in ovarian cancer cell lines is overcome by co-treatment with cytotoxic drugs
Tomek et al.; Gynecol Oncol. 2004 Jul;94(1):107-14
Nitrite-mediated protection against hypochlorous acid-induced chondrocyte toxicity. A novel cytoprotective role of nitric oxide in the inflamed joint?
Whiteman et al.; Arthritis Rheum. 2003 Nov;48(11):3140-50

To view questions and answers for this product, please click here.
http://www.promocell.com/knowledge-base/search-for-information/?tx_na15knowledgebase_pi1%5Bsword%5D=HCH&tx_na15knowledgebase_pi1%5Bcategories%5D%5B0%5D=3&tx_na15knowledgebase_pi1%5Bcategories%5D%5B1%5D=1&tx_na15knowledgebase_pi1%5Bcategories%5D%5B2%5D=2&tx_na15knowledgebase_pi1%5Bresults_at_a_time%5D=30
 
 
 
                    PromoCell技术支持
                    电话：021-68369210
                    QQ：2788626247
                      普诺生生物技术有限公司