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BSA, in concentrations of up to 0.8µg/µl (higher than previously described) appeared to increase the efficiency of the PCR reaction much more than either DMSO or glycerol. It should be noted that BSA did not have an inhibitory effect on any of the loci amplified (data not shown). Fig. 42. Comparative multiplex PCR using mixtures A to D with 5% DMSO (superscript D) and without DMSO, in 1x buffer. Some loci from mixture A (blue arrows) are stronger when no DMSO is used. However, DMSO helps amplify (magenta arrows) one locus in mix B and one locus in mixture D. Amplification of PCR products of mixture C* were unaffected by DMSO. PCR方法相关产品:Various authors recommend DMSO and glycerol to improve amplification efficiency (higher amount of product) and specificity (no unspecific products) of PCR, when used in concentrations varying between 5-10% (v/v). In the multiplex reaction, however, these adjuvants gave conflicting results. For example, 5% DMSO (Fig. 42) improved the amplification of some products, decreased the amount of others whereas some loci were not influenced at all. Similar results were obtained with 5% glycerol (data not shown). Therefore, the usefulness of these adjuvants needs to be tested in each case. PCR/RT-PCR/qPCR试剂PCR引物 PCR试剂PCR对照特异性PCR试剂盒PCR克隆试剂盒RNA RNase检测/去除 RT-PCR试剂 RT-PCR标准品 定量PCR试剂 定量PCR标记 总RNA分离纯化盒PCR产物纯化核酸酶 聚合酶 反转录酶