Wash cells with HBSS Scrape remaining cells, pellet and flash freeze for protein/RNA analysis Stain cells for beta-gal expression: Fixing Solution : 1.35 ml 37% Formaldehyde 0.2 ml Gluteraldehyde 1X PBS to 25 mls Staining Solution: 625 l 40 mg/ml X-gal in DMF 50 l 1M MgCL2 750 l 100 mM Potassium Ferricyanide 750 l 100 mM Potassium Ferrocyanide 1X PBS to 25 mls [100 mM Potassium Ferrocyanide = 2.11 g/50ml PBS] [100 mM Potassium Ferricyanide = 1.64 g/50 ml PBS] Store at room temperature, protect from light. 1. Make -gal solution and warm in 37 C water bath to prevent crystallization 2. Aspirate off media 3. Wash cells 1X PBS 4. Fix cells 5 min at RT in fixing solution 5. Wash 3X PBS 6 Optional: Add PBS to cells and warm plate at 37 C 5 min (reduces crystallization) 7. Stain cells with staining solution at 37 C for 1-24 hrs 8. Store stained cells in 70% EtOH at 4 C (EMBO J. 5: pg 3133, 1986) 出品公司: Imgenex 载体名称: pCL-Ampho, pCL Ampho 质粒类型: 哺乳动物载体;逆病毒包装;单质粒包装系统 高拷贝/低拷贝: 高拷贝 克隆方法: 限制性内切酶,多克隆...pCL-Ampho,逆病毒包装质粒,病毒包装,包装载体,序列,价格,方法,流pCL-Ampho逆病毒包装载体质粒价格1250元，pCL-Ampho载体质粒图谱(Vector map)，载体序列见下文，载体质粒抗性Ampicillin。逆病毒包装质粒pCL-Ampho载体是单质粒系统逆病毒包装载体使用方法见下文。pCL
