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蚂蚁淘(苏州)生物科技有限公司TheINCOGNITO™T7-RNATranscriptionKit*isoptimizedforhigh-yieldsynthesisofpseudouridinecontainingRNA(-RNA)byinvitrotranscription(IVT)ofaDNAtemplatethathasaproperly-orientedphageT7RNApolymerasepromoterusingT7-Scribe™RNApolymerase,thecanonicalnucleotidesATP,CTP,GTP,andthemodifiednucleotidepseudouridine-5"-triphosphate(TP).AlthoughyieldvarieswiththeDNAtemplateandotherfactors,astandard20-μlIVTreactionusing1μgofacontroltemplateDNAyieldsapproximately100μgof-RNAin2hoursat37oC.The-RNAcanbeenzymatically5"-cappedand3"poly(A)-tailedusingCELLSCRIPT"sScriptCap™m7GCappingSystem,ScriptCap™2"-O-MethyltransferaseandA-Plus™Poly(A)Polymerasetogenerate mRNAforexpressionincells.Ithasbeenshownthat-mRNAsaretranslatedintoproteinathigherlevelsandinducelowerinnateimmuneresponsesinhumanandothermammaliancellsthatexpressvariousRNAsensorscomparedtocorrespondingunmodifiedmRNAs.
CELLSCRIPTC-ICTY110510品牌名称:
CELLSCRIPT
CELLSCRIPTC-ICTY110510品牌logo:
CELLSCRIPTC-ICTY110510品牌官网:
http://www.cellscript.com
CELLSCRIPTC-ICTY110510品牌介绍: CELLSCRIPT专注于再生医学,细胞再生学以及细胞疗法。细胞、组织或器官的移植治疗及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的
合成修饰mRNA。
Themissionof CELLSCRIPT™ istoprovidethebestproductsandtechnologiesformakingandusingRNAfortranslationincellsforclinicalresearchandtherapeutics.
Currentproductsincludekitsfor invitro transcription,5"RNAcappingusingeitheracapanalogorcappingenzymes,and3"RNApolyadenylation,aswellasall-in-onekitsformakingcapped,poly(A)-tailedmRNAfortranslationincells.
CELLSCRIPT™ recentlyintroducedINCOGNITO™RNATranscriptionKitsfor invitrosynthesisofRNAthatcontainsmodifiednucleosides,suchaspseudouridine(Ψ)and/or5-methylcytidine(m5C)inplaceofthecorrespondingUorCcanonicalnucleosides(Figure1).Thesekitsareso-namedbecausethecapped,polyadenylatedandnucleoside-modifiedRNAproducts(calledINCOGNITOmRNAs)aredisguisedsotheydonotinduceinnateimmuneresponsestothesameextentasthecorrespondingunmodifiedmRNAswhentransfectedintomammaliancellsthatexpressavarietyofRNAsensors. No.品牌货号名称规格1CELLSCRIPTC-ACM04037AmpliCap-Max™T7HighYieldMessageMakerKit25rxns2CELLSCRIPTC-AC0706AmpliCap™SP6HighYieldMessageMakerKit25rxns3CELLSCRIPTC-C50110Anti-ReverseCapAnalog(ARCA)500nmol4CELLSCRIPTC-CC15011HCap-Clip™AcidPyrophosphatase100U5CELLSCRIPTC-ICSY110510INCOGNITO™SP6Ψ-RNATranscriptionKit10rxns6CELLSCRIPTC-MSC100625T7mScript™StandardmRNAProductionSystem25rxns7CELLSCRIPTC-MMA60710MessageMAX™T7ARCA-CappedMessageTranscriptionKit10rxns8CELLSCRIPTC-SRI6310KScriptGuard™RNaseInhibitor10,000U9CELLSCRIPTC-SCMT0610ScriptCap™2"-O-MethyltransferaseKit10rxns10CELLSCRIPTC-ICTY110510INCOGNITO™T7Ψ-RNATranscriptionKit10rxns11CELLSCRIPTC-MMA60710MessageMAX™T7ARCA-CappedMessageTranscriptionKit10rxns12CELLSCRIPTC-C61025Monomethylated RNACapAnalog1,250nmol13CELLSCRIPTC-PRM101125PseudoU-RNAMolecularWeight
Markers25lanes14CELLSCRIPTC-AS3106SP6-Scribe™StandardRNAIVTKit50rxns15CELLSCRIPTC-PAP5104HA-Plus™Poly(A)PolymeraseTailingKit50rxns
CELLSCRIPTC-ICTY110510联系方式:
联系人:谢先生
400电话:4009658633
办公电话:010-56256916
QQ:3212874516,3198592576
网址:http://www.annoron.com/
CELLSCRIPTC-ICTY110510产品相关链接:
1ADPP/N384
2Collagen P/N385
3ThrombinP/N386
4ArachidonicAcidP/N390
5EpinephrineP/N393
供应商:蚂蚁淘(苏州)生物科技有限公司
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