THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

TABLE OF CONTENTS

1. Abstract

2. Introductory statement

3. The key preparatory steps

3.1. Fixative

3.1.1. Protease digestion

3.1.2. Definition of optimal protease digestion

3.1.3. Definitions of suboptimal and over-digestion

3.1.4. DNase digestion

3.1.5. Direct incorporation of the reporter nucleotide

4. A one step protocol for RT in situ PCR

5. Applications of RT in situ PCR Matrix metalloprotease expression in cancer as a model for RT in situ PCR

5.1. MMP and TIMP expression in cervical cancer

6. Concluding remarks

7. References

8. Entire manuscript