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一、ProteaseInhibitorCocktail,mini-Tablet(EDTA-Free)，货号HY-K0011：
1.Description
Endogenousproteinsareproducedandremovedinabalancedstate,sotheircellularlevelsaregenerallystableunderstableenvironmentalconditions.However,proteinproductionisgreatlyhaltedanddegradationisenhancedwhencellsarestudiedinvitro.Topreventthedegradationofproteinsundersuchconditions,onecanutilizeacocktailofsmallmoleculeinhibitorstoblocktheactionofproteases.Thecocktailfunctionstoinhibitproteasesthatwoulddegradeeitherphosphorylatedornon-phosphorylatedproteinsubstrates.
MedchemexpressProteaseInhibitorCocktail(EDTAfree,mini-Tablet)isablendof5pan-proteaseinhibitorsforprotectionofproteinintegrity.Eachcomponenthasspecificinhibitoryproperties.AEBSFandAprotininacttoinhibitserineproteases,includingtrypsin,chymotrypsin,andplasminamongstothers.Bestatininhibitsaminpeptidases.E-64actsagainstcysteinproteases.Leupeptinactsagainstbothserineandcysteinproteases.
2.ComponentsProtocol
1)ThisproductcanbeappliedinWesternBlotanalysis,Co-IP,pull-down,IF,IHC,kinaseassayandetc.
2)Onemini-Tabletcanbeaddedto10mLextractionmediumdirectly.Alternatively,astocksolution(10Xconc.onemini-Tabletin1mLddH2O)canbeprepared.Vortexbrieflytoacceleratethedissolution.Thestocksolutionisstableforoneweekwhenstoredat4°C,andforatleast4weeksat-20°C.
3.Components
4.Storage/StABIlity
ProteaseInhibitorCocktail(EDTAfree,mini-Tablet)canbestoredat4°Cfor2years.
5.Notice
1)Forfasterdissolution,vortexbrieflyafteraddingthemini-TabletintotheextractionmediaorddH2O.
2)SomeDUBproteases(oneexampleisATAXIN-3)cannotbesuppressedbytrADItionalproteaseinhibitors,suchasE-64,AEBSF,bestatin,leupeptinandAprotinin.(Reference:NeilD.Rawlings,GuySalvesenetal.HandbookofProteolyticEnzymes,Vol.1,2012).
6.PrecautionsandDisclaimer
ThisproductisforR&Duseonly,notfordrug,household,orotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
二、ProteaseInhibitorCocktail(EDTA-Free,100XinDMSO)，货号HY-K0010：
1.Description
Endogenousproteinsareproducedandremovedinabalancedstate,sotheircellularlevelsaregenerallystableunderstableenvironmentalconditions.However,proteinproductionisgreatlyhaltedanddegradationisenhancedwhencellsarestudiedinvitro.Topreventthedegradationofproteinsundersuchconditions,onecanutilizeacocktailofsmallmoleculeinhibitorstoblocktheactionofproteases.Thecocktailfunctionstoinhibitproteasesthatwoulddegradeeitherphosphorylatedornon-phosphorylatedproteinsubstrates.
MedChemExpressProteaseInhibitorCocktail(EDTAfree,100XinDMSO)isablendof6pan-proteaseinhibitorsforprotectionofproteinintegrity.Thismixturecontainsindividualcomponents,includingAEBSFat104mM,Aprotininat80μM,Bestatinat5mM,E-64at1.5mM,Leupeptinat2mMandPepstatinAat1.5mM.Eachcomponenthasspecificinhibitoryproperties.AEBSFandAprotininacttoinhibitserineproteases,includingtrypsin,chymotrypsin,andplasminamongstothers.Bestatininhibitsaminpeptidases.E-64actsagainstcysteinproteases.Leupeptinactsagainstbothserineandcysteinproteases.PepstatinAinhibitsacidproteases.
2.Protocol
1)ThisproductcanbeappliedinWesternBlotanalysis,Co-IP,pull-down,IF,IHC,kinaseassayandetc.
2)Thawatroomtemperature,addat1:100(v/v)dilutionto1solutionsamples(suchascelllysatesortissueextracts),beforeassaying.Onetubeisrecommendedforinhibitionofproteasespresentincell.
3)Extractsofupto20gwetweight.
3.Components
4.Storage/Stability
ProteaseInhibitorCocktail(EDTAfree,100X)canbestoredat-20°Cfor12months.
5.Notice
1)DMSOsolutionthawsslowlyinlowtemperature.Recommendtowarmupat25°C.
2)Notalllysatescontainthesamelevelsofendogenousenzymes,anditmaybenecessarytoadjustthevolumeofcocktailrequired.
6.PrecautionsandDisclaimer
ThisproductisforR&Duseonly,notfordrug,household,orotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
北京乐博生物联系方式：
全国免费咨询热线：4006130504
电话：010-50934026/50934036/52405563
传真：010-58850899
邮箱：info@lab-bio.com
网站：www.lab-bio.com
地址：北京市海淀区清河三街95号同源大厦912室