SuperSW mAb HTP色谱柱在HILIC模式下分离核酸碱基（AN64）

Introduction
HydrophilicInteractionLiquidChromatography(HILIC)isoneofthefastestgrowingmodesofseparation,inwhichanypolarchromatographicsurfacecanbeused.Chemicallybondeddiolcoatedphases,asfoundinTSKgelSWsizeexclusionchromatography(SEC)columns,demonstratehighpolarityandhydrogenbondingpropertiesanddonotcontainionizablegroupsotherthantheunreactedresidualsilanols,makingthemappropriateforHILICmode.

Formanyyears,SECcolumnshavebeenusedtoseparatevariousnucleicacidspeciessuchasDNA,RNAandtRNAaswellastheirconstituentbases,adenine,guanine,thymine,cytosine,anduracil.Inmedicine,severalprimarynucleobasesarethebasisforthenucleosideanaloguesandothersyntheticanalogswhichareusedasanticancerandantiviralagents.Nucleobasemodificationsarethebasisofoligonucleotide-basedtherapeutics,makingtheir
purificationveryimportant.

TheTSKgelSuperSWmAbHTPcolumnisanewlyintroducedSECcolumndesignedforthehighthroughputseparationofmonoclonalantibodiesfromtheirhighandlowmolecularmassvariants.TSKgelSuperSWmAbHTPhasadiolcoatingtominimizesecondaryinteractionswhichmayoccurinSECseparations.ThisnotedemonstratesthebenefitsofusingaTSKgelSuperSWmAbHTPcolumninHILICmodeforthesuperiorresolutionoffournucleobases,asopposedtousingthecolumninSECmodeorusingaHILICcolumn.