R&D Systems/Human Kallikrein 2 Antibody/MAB4104/100 ug

• Species Reactivity
  Human
• Specificity
  Detects human Kallikrein 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or B1 is observed.
• Source
  Monoclonal Mouse IgG₁ Clone # 426723
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human Kallikrein 2
  Ile25-Pro261
  Accession # P20151
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  Recombinant Human Kallikrein 2 (Catalog # 4104-SE).
• Immunoprecipitation
  25 µg/mL
  Conditioned cell culture medium spiked with Recombinant Human Kallikrein 2 (Catalog # 4104-SE), see our available Western blot detection antibodies
• Neutralization
  Measured by its ability to neutralize Recombinant Human Kallikrein 2 (2 µg/mL, Catalog # 4104-SE) cleavage of the fluorogenic peptide substrate PFR-AMC (0.1 mM). The Neutralization Dose (ND₅₀) is typically 3.4 µg/mL.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
  2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.
• Entrez Gene IDs:
  3817 (Human); 24841 (Rat)
• Alternate Names:
  EC 3.4.21; EC 3.4.21.35; glandular kallikrein 2; Glandular kallikrein-1; hGK-1; hK2; Kallikrein 2; kallikrein 2, prostatic; kallikrein-2; kallikrein-related peptidase 2; Klk1c2; KLK2; KLK2A2; MGC12201; Tissue kallikrein-2; Ton