R&D Systems/Human Stabilin-2 Antibody/AF3645/100 ug

• Species Reactivity
  Human
• Specificity
  Detects human Stabilin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Stabilin-1 is observed.
• Source
  Polyclonal Sheep IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human Stabilin-2
  Gly2198-Val2295
  Accession # Q8WWQ8
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Human Stabilin‑2 (Catalog # 3645-S2)
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Intracellular Staining by Flow Cytometry
  2.5 µg/10⁶ cells
  See below

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Politz, O. et al. (2002) Biochem. J. 362:155.
  2. Zhou, B. et al. (2003) Glycobiology 13:339.
  3. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  4. Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
  5. Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
  6. Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
  7. Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
• Entrez Gene IDs:
  55576 (Human); 192188 (Mouse); 282580 (Rat)
• Alternate Names:
  CD44-like precursor FELL; DKFZP434E0321; FAS1 EGF-like and X-link domain containing adhesion molecule-2; FAS1 EGF-like and X-link domain-containing adhesion molecule 2; fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2; Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2; FEEL2; FEEL-2; FELE-2; FELL; FELL-2; FEX2; HARE; HAREFELL2; hepatic hyaluronan clearance receptor; Hyaluronan receptor for endocytosis; hyaluronic acid receptor for endocytosis; STAB2; STAB-2; stabilin 2; Stabilin2; Stabilin-2

Background:

Stabilin-2, also known as HARE (hyaluronan receptor for endocytosis) and FEEL-2 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 2), is a type I transmembrane multi-domain protein that is the closest homolog of stabilin-1 (1, 2). It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG-6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin-2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin-coated pits (4). As a recycling receptor, Stabilin-2 cycles from the membrane to clathrin-coated pits each 10‑15 minutes, with about two thirds of the protein found within the endocytic system at any one time (4). The link domain binds and mediates the systemic clearance of hyaluronan (HA) (2‑6). Within the link domain (aa 2198‑2295), human Stabilin-2 shares 86% and 89% aa identity with mouse and rat Stabilin-2, respectively. Total human HA is about 15 grams, of which about 5 grams are cleared each day (5). Stabilin-2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end-product (AGE), collagen N-terminal propeptides and acetylated LDL (4‑7). Human Stabilin-2 mRNA encodes a 2251 aa, 315 kDa protein that produces an isoform of 190 kDa through proteolytic cleavage (2, 6). The 190 kDa form lacks some N-terminal fasciclin and EGF-like domains, but shows similar activities when compared with the 315 kDa form (2, 6).