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Excellgen/Thermolabile Uracil-DNA Glycosylase, UDG/EG-1015/100 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Excellgen/Thermolabile Uracil-DNA Glycosylase, UDG/EG-1015/100 Units

Description

ThermolABIleUracil-DNAGlycosylase(ThermolabileUDG)removesuracilfromsingle-strandedordouble-strandedDNAbyhydrolyzingtheN-glycosylicbondbetweenthedeoxyriboseandthebaseleavinganAP(apurinicorapyrimidinic)site.Thisenzyme(1-10units)issensitivetoheatandcanbecompletelyinactivatedbya10minuteincubationattemperaturesabove50°C.

Applications
  • PreventionofCarry-overContaminationinPCR
  • RemoveUracil-basefromDNA
SourceAnE.colistrainthatcarriesanUDGgenefromamarinebacterium
UnitDefinition1unitisdefinedastheamountofThermolabileUDGrequiredtorelease1nmolofUracilfromdU-containingDNAinonehourat37°C.
Components
  • ThermolabileUDG:1,000units/mlin50mMTris-HCl,50mMNaCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C
  • 10XReactionBuffer:700mMTris-HCl,100mMNaCl,10mMEDTA,1mg/mLBSA,pH8.0@25°C
QualityControl

Theabsenceofsingleanddouble-strandexonuclease,andendodeoxyribonucleasesisconfirmedbyappropriatequalitytests

StorageCondition

-20°C

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