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DescriptionLeavesomefeedback! TestedandCertifiedforinvivoimaging(See"LuciferinFAQ"inadditionalinformation)Luciferinisacommonbioluminescentreporterusedforinvivoimagingoftheexpressionofluciferase.ThiswatersolublesubstrateforthefireflyluciferaseenzymeutilizesATPandMg2+ascofactorstoemitacharacteristicyellow-greenemissioninthepresenceofoxygen,whichshiftstoredlightinvivoat37°C.ThroughtheutilizationofATP,thereactioncanbefurtherusedtoindicatethepresenceofenergyorlifeinordertofunctionasalife-deathstain.Luciferinisacommonreagentusedthroughoutthebiotechnologyfieldandspecificallyforinvivoimaging.Luciferaselabeledtumorcells,stemcellsorinfectiousdiseasesareofteninoculatedintoresearchanimalssuchasratsormiceforinvestigation.Theinjectionofluciferinallowsforthereal-time,noninvasivemonitoringofdiseaseprogressionand/ordrugefficacyinthesemodelsystemsthroughBioluminescenceImaging(BLI).Luciferinisalsocommonlyusedforinvitroresearch,includingluciferaseandATPassays,genereporterassays,highthroughputsequencingandvariouscontaminationassays.FireflyLuciferinisidenticaltoBeetleLuciferin.(See"AdditionalInformation"forstructures.)ProductSpecifications:D-Luciferin,PotassiumSalt,4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacidpotassiumsalt,MOLECULARBIOLOGYGRADE(PremiumPure)Formula:KC11H7N2O3S2MW:318.42g/molColor:LightyellowpowderPurity:>99%pure.(QualityverifiedbynineindependentcriteriaincludingHPLCandFTIR.)Storage/Handling:Storedesiccatedat-20°C.Protectfromlight.PubChemChemicalID:44134804
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