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Excellgen/Nuclear receptor coactivator 1, SRC1-RID (His/TEV)/RP-134/10,000 U188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Excellgen/Nuclear receptor coactivator 1, SRC1-RID (His/TEV)/RP-134/10,000 U

ProductNameNuclearreceptorcoactivator1,SRC1-RID(His/TEV)
Size10,000U
DescriptionSteroidreceptorcoactivator1(SRC1)isatranscriptionalcoactivatorthatmediatestheactivatingfunctionsofmanyofthenuclearhormonereceptors.ItisalsoknownasNCoA1andisamemberoftheSRC/p160coactivatorfamily(1).SRC1hasbeenshowntobeoverexpressedinsomecancers(2).SRC1isa160kDaproteinthatcontainsseveralLXXLLmotifs,whichareinvolvedinnuclearreceptorinteraction(3).Theregion627-786contains3LXXLLmotifsthatareinvolvedininteractionwithnuclearhormonereceptors(4,5)andhavebeenpreviouslyusedinassaysdetectingligand-dependentreceptor-cofactorinteractions(6).ThisproteincontainstheTobaccoEtchvirus(TEV)proteasesiteandcanbecleavedusingacTEVtoyielduntaggedSRC1RID.Thisproteinwillbeusefulforassayswhereanativeuntaggedproteinisdesired
Applicationsprotein-proteininteractionassays
SourceE.coli
FusionTag6XHis(canberemovedbyTEV)
PurificationMethodFPLC
UnitDefinition(Activity)1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay.
Purity

~95%asdeterminedbySDS-PAGE

GeneID8648
Accession#NM_003743
GeneNameNuclearreceptorcoactivator1
GeneSymbolNCOA1
GeneSynonymsSRC1;KAT13A;RIP160;F-SRC-1;bHLHe42;bHLHe74
MW
ProteinSequenceSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTER
HKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDH
QLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNT
StorageBuffer20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol
Storage-20to-80°C.
Shipping4°Cordryice
References1.Onate,S.etal.,(1995)Science270,1354-1357
2.Yanase,T.,etal.,(2004)Intern.Med.43,368-373
3.Heery,D.etal.,(1997)Nature387,733-736
4.Xu,J.andLi,Q.,(2003)Mol.Endocrinol.17,1681-1692
5.Bai,Y.andGiguere,V.,(2003)Mol.Endocrinol.17,589-599
6.Cho,M.etal.,(2003)J.Biochem.Mol.Biol.36,207-213
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