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Excellgen/Bst DNA Polymerase, large fragment, 100 U/µl/EG-1084/20,000 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Excellgen/Bst DNA Polymerase, large fragment, 100 U/µl/EG-1084/20,000 Units

Description

RecombinantBstDNAPolymerase(largefragment)has5’->3’polymeraseactivity,butlacks5’->3’exonucleaseactivity.ThisenzymecanbeusedforandrapidamplificationoftraceamountsofDNAtemplatesforsequencing.

Applications
  • DNAsequencingthroughhighGCregions
  • RapidamplificationoftraceamountsofDNAtemplates
  • NextGenerationsequencing
SourceArecombinantE.colistraincarryingthelargefragmentofDNApolymerasegenefromBacillusstearoThermophilus
UnitDefinitionOneunitisdefinedastheamountofpolymeraserequiredtocatalyzetheincorporationof10nmolofdNTPintoacid-insolublematerialin30minutesat65°C.
Components
  • BstDNAPolymerase:100,000U/mlin10mMTris-HCl,50mMKCl,1.0mMDithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%Glycerol,pH7.5@25°C
  • 10XReactionBuffer:200mMTris-HCl,100mM(NH4)2SO4,100mMKCl,20mMMgSO4,and1.0%TritonX-100,pH8.8@25°C
QualityControlTheabsenceofendonuclease,nickingactivityandexonucleaseactivityisconfirmedbyappropriatequalitytests.
StorageCondition

-20°C

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