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| ProductName | PC4(PositiveCofactor4wildtype)TranscriptionalCoactivator |
| Size | 10,000U |
| Description | HumanPC4isanon-TAFtranscriptioncoactivatorthatmediatesactivator-dependenttranscriptionbyRNApolymeraseIIinvitrothroughmosttestedactivators.ThefunctionofPC4isapparentlythroughinteractionswithtranscriptionalactivatorsandthebasaltranscriptionmachinery.ItisnegativelyregulatedbycaseinkinaseIIphosphorylationbothinvitroandinvivo(1-3).PC4stronglybindssinglestrandedDNAandregulatesHSSB(RPA)-dependentSV40DNAreplication(4,5).RecentstudiesindicatedthatPC4canbeacetylatedbyseveralhistoneacetyltransferases(6). |
| Applications | Transcription,DNAreplication,invitrophosphorylation,gelmobilityshiftassay,protein-proteininteractionsandasasubstrateforinvitroacetylation. |
| Source | E.coli |
| FusionTag | None |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 10923 |
| Accession# | NM_006713 |
| GeneName | SUB1homolog(S.cerevisiae) |
| GeneSymbol | SUB1 |
| GeneSynonyms | P15;PC4;p14 |
| MW | 14.4kDa |
| ProteinSequence | MPKSKELVSSGSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRD DNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDI DDAVRKL |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Ge,H.etal.,(1994)Cell78,513-523;(1994)Proc.Natl.Acad.Sci.USA91,12691-12695 2.Kretzschmar,M.etal.,(1994)Cell78,525-534 3.Ge,H.etal.,(1996)MethodsEnzymol.274,57-71 4.Ballard,DW.etal.,(1988)J.Biol.Chem.263,8450-8457 5.Pan,ZQ.etal.,(1996)J.Biol.Chem.271,22111-22116 6.Kumar,BR.etal.,(2001)J.Biol.Chem.276,16804-1689 |
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