Y9140LCL,Enzymatics,DNA Modifying Enzymes,Enzymatics,+,DNA修饰酶,Array.Array.Array蚂蚁淘厂家直采.

Enzymatics,inc 主营：连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
产品中心DNA Modifying EnzymesEnzymatics/End-Repair Mix (Low Concentration)/Y9140-LC-L/200 Reactions
Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer servi...
End-RepairMix(LowConcentration)ProductDescription

TheEnd-RepairMixconvertsDNAcontainingdamagedorincompatIBLe5′-and/or3′-protrudingendsto5′-phosphorylated,blunt-endedDNA.Thislow-concentrationformulationoftheEnd-RepairMixiscompatiblewithapplicationsrequiring 1microgramofDNAtobepreparedforblunt-endligation.Theconversiontoblunt-endedDNAisaccomplishedbyexploitingthe5′→3′polymeraseand3′→5′exonucleaseactivitiesofT4DNAPolymerase(P7080).T4PolynucleotideKinase(Y9040)ensuresthattheendsoftheblunt-endedDNAfragmentsare5′-phosphorylatedforsubsequentligationbyT4DNALigase(L6030-HC).

SourceofProtein
Purifiedfromstrainsof E.coli thatexpresstherecombinantT4DNAPolymerase,andT4PolynucleotideKinasegenes,respectively.

Suppliedin
100mMKCl
10mMTris-HCl
0.1mMEDTA
1mMDTT
0.1%TritonX-100
50%glycerol
pH7.4@25°C

SuppliedWith
B9140(10XEnd-RepairBuffer)
N2060(1mMdNTPs)

10XEnd-RepairBuffer(B9140):
1MTris-HCl
500mMNaCl
100mMMgCl2
50mMDTT
0.25%Triton-X100
pH7.5@25°C

ContaminationTests

Purifiedfreeofcontaminatingendonucleases.Inaddition, 99%enzymepurityisanalyzedbySDS-PAGE,andnegligible E.coli genomicDNAisconfirmedbyqPCR.

FunctionalAssay
2µLofEnd-RepairMixwasaddedtoadoublerestrictionenzymedigested,dephosphorylatedplasmidDNAin1Xreactionbuffercontaining0.1mMdNTPsandincubatedat25°Cfor30minutes.Competentcellsweretransformedwiththeligationmixure,platedontoLB/Amp/X-Galplatesandincubatedovernightat37°C.ControlreactionsconsistingofEnd-RepairMixwithoutT4DNApolymeraseand/orT4PolynucleotideKinaseweretestedinparallel.TheefficiencyofthereactionwasevaluatedbycomparingthenumberofblueandwhitecoloniespresentintheEnd-RepairMixplatestothoseofthecontrolplates.

ViewPDFPosterInstructionsFAQProductInformationEnd-RepairMix(LowConcentration)PartNumberY9140-LC-LPrice$363Concentration1Rxn/µLUnitSize200ReactionsSDSAvailableonrequestProductSpecification*StorageTemperature-25to-15°CTestSpecificationPurity(SDS-PAGE) 99%3′→5′NucleaseFunctional5′PhosphorylationFunctional5′→3′DNASynthesisFunctionalDSEndonuclease10µL=NoconversionE.coliDNAContamination10µL 10copiesUsageInstructionsPurifyDNAtobeblunted,dissolveinTEbuffer.Combineandmixthefollowingcomponentsinasteriletube:
1-19µLPurifiedDNA(upto1.0µg)
2.5µL10XEnd-RepairBuffer
2.5µL1mMdNTPmix(N2060)
1-3µLEnd-RepairEnzymeMix
SterileH2Oto25µL
TotalVolume:25µLIncubateroomtemperature(25°C)30minutes.InactivateEnd-RepairEnzymebyheatat75°Cfor20minutes.LigationmaybeperformedimmediatelyusingEnzymaticsRapidformatT4DNALigase(L6030-HC).Notes

ATPisnotrequiredbecausetheT4PolynucleotideKinasecanutilizethedeoxynucleotides(dATPanddTTP)usedinthereactionasphosphatedonors.

LimitationsofUse
Thisproductwasdeveloped,manufactured,andsoldforinvitrouseonly.TheproductisnotsuitableforadmiNISTrationtohumansoranimals.SDSsheetsrelevanttothisproductareavailableuponrequest.

Enzymatics is an emerging leader in the supply of the enzymes that drive nucleic acid detection technologies. Our leadership is currently building a team which embodies our mission to lead the molecular biology tools market in quality, customer service, and innovation. We seek engaging, motivated individuals who can articulate their goals and, in turn, offer a culture that breeds success. Enzymatics is an employee-focused organization that strives to cultivate challenging careers and rewarding lives. If you seek a challenge, have a thirst for knowledge, and are interested in having fun while working, please apply to learn more! We offer a competitive benefits package and an incentive-based compensation package.

苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司，以常规生物学实验外包与生物学试剂为基础，放眼于生命科学领域的前沿技术服务，公司技术骨干有着多年的生物学科研背景，毕业于国内外名牌大学，并且有着丰富的项目经验，了解国内外科研行情，我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点，和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括：抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人，认真做事，服务至上“，希望用我们的专业知识和项目经验为国内科研客户解决科研问题，祝您科研之路畅通无阻！

恭喜蚂蚁淘获得Enzymatics公司代理授权 蚂蚁淘(ebiomall）是一家生命科学领域的垂直电商平台，公司专注生物医学科研用品的 全球导购和品牌推广，为海外厂方与国内代理经销商搭建跨境贸易系统和交流平台。 产品齐全，200+分类，1000+品牌，500万+产品信息。100%正品，专业售后，到货快，在线 下单， 简单轻松，节省科学家宝贵的科研时间。 借助互联网平等而开放的力量，矢志实现生命科学领域全球的信息同步，我们相信一群充满梦想的人，可以凭借着专业与执着，让你的购物更轻松！ 2019年蚂蚁淘再获得英国TwistDx公司代理授权 订货请咨询苏州蚂蚁淘生物科技有限公司 关于Enzymatics Qiagen旗下的Enzymatics品牌专注于酶产品的研发，其生产的高品质酶类产品促进了核酸检测技术的发展，使全球客户变革了诊断、治疗和生命科学的研究。业务范围包括：试剂、供应链解决方案，应用科学等。其测序产品市场占有率高达80%。 超纯连接酶(如T4连接酶)、超纯poly酶、Archer™系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。满足用户丰富多样的研究需求。 Enzymatics的酶产品被广泛应用于生命科学、临床应用领域二代测序和其他遗传分析技术领域。