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T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1). T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities (2, 3, 4, 5, 6).
Source of ProteinPurified from a strain of E.coli that expresses the recombinant T4 Polynucleotide Kinase gene.
Supplied in10 mM Tris-HCl50 mM KCl0.1 µM ATP1.0 mM DTT0.1 mM EDTA50% glycerolpH 7.4 @ 25°C
Supplied WithB9040 (10X T4 Polynucleotide Kinase Buffer)
10X Polynucleotide Kinase Buffer (B9040)700 mM Tris-HCl100 mM MgCl250 mM DTTpH 7.6 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
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