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Exonuclease I cleaves single-stranded DNA in the 3′→5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5′-terminus intact. The enzyme is processive though digestion is inhibited by the presence of a 3′ -terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium (1-3).
Source of ProteinPurified from a strain of E. coli that expresses the recombinant Exonuclease I gene.
Supplied in10 mM Tris-HCl100 mM NaCl1 mM DTT0.5 mM EDTA50% glycerolpH 7.5 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.
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