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| ProductName | PartNo. | UnitSize | Concentration | Notes | |
| NEWPRODUCTS: | |||||
| E.coliDNALigase | L6090L | 2,500 | 10,000U/ml | EfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG | |
| T4RNALigase2 | L6080L | 4,500 | 30,000U/ml | LigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures | |
| T4RNALigase2Truncated | L6070L | 500 | 5,000U/ml | Requiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA | |
| OmniKlentaq® | P7500-HC-L | 8,400 | 42,000U/ml | EngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes | |
| (HighConcentration) | |||||
| OmniKlentaq® | P7500-LC-L | 8,400 | 4,200U/ml | Lowerconcentration–1microliterperreaction | |
| (LowConcentration) | |||||
| PhoenixHotStartTaqDNAPolymerase | P7590L | 500 | 5,000U/ml | AntibodybasedHotStartTaqDNApolymerasedeliversrobustPCRperformancewithexceptionalpre-PCRcyclingroomtemperaturestABIlity | |
| VeraSeq™2.0High-FidelityDNAPolymerase | P7511L | 500 | 2,000U/ml | Industry-leADIngspeed,fidelityandrobustnessdeliveredviaanengineeredPCRpolymerase | |
| VeraSeq™ULtraDNAPolymerase | P7520L | 500 | 2,000U/ml | Highfidelity,speed,androbustnessinPCRdeliveredbyanengineered,ThermostableDNApolymerasethattoleratesuraciltemplates,nucleotidesandprimers | |
| RecA | Y9260L | 1.5mg | 2.0mg/ml | Promotesstrandexchangeofsingle-strandedDNAfragmentswithDNAduplexsubstrates | |
| ThermolabilePhosphatase | Y9210L | 5,500 | 5,000U/ml | Removes5′and3′-phosphategroupsfromDNA | |
| ThermolabileUDG | G5020L | 500 | 1,000U/ml | RemovesuracilfromDNA,leavingAPsite | |
| PolyAPolymerase | P7460L | 1,000 | 5,000U/ml | CatalyzestheadditionofAMPfromATPtothe3′hydroxylofRNAwhichisusefulformakingpolyAtailedRNA | |
| UltraPureLigases: | |||||
| Bitmap E.coliDNALigase | L6090L | 2,500 | 10,000U/ml | EfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG | |
| T3DNALigase | L6010L | 900,000 | 3,000,000U/ml | Worksinhighionicstrength–to1.0MNaCl | |
| T4DNALigase | L6030-LC-L | 150,000 | 120,000U/ml | Lowconcentrationovernightligationformat | |
| T4DNALigase(Rapid) | L6030-HC-L | 240,000 | 600,000U/ml | “Ultrapureligase”referencedinNatureMethods(Quail,1December2008) | |
| Bitmap T4RNALigase1 | L6050L | 10,000 | 20,000U/ml | Single-strandedRNAligase.Alsojoinssingle-strandedDNAmolecules | |
| T4RNALigase2 | L6080L | 4,500 | 30,000U/ml | LigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures | |
| Bitmap T4RNALigase2Truncated | L6070L | 500 | 5,000U/ml | Requiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA | |
| T7DNALigase | L6020L | 900,000 | 3,000,000U/ml | 1000foldhigheractivityonstickyendsthanbluntends | |
| TaqDNALigase | L6060L | 20,000 | 40,000U/ml | ThermostableDNAligasewhichefficientlysealsnicksanddiscriminatesagainstmismatchligation | |
| UltraPurePolymerases: | |||||
| DNAPolymeraseI | P7050L | 5,000 | 10,000U/ml | DNApolymerasewith5′→3′synthesis, | |
| 5′→3′and3′→5′exonucleaseactivities | |||||
| Klenow(3′→5′exo-) | P7010-HC-L | 10,000 | 50,000U/ml | ProvenchoiceforDNAlabeling,A-tailing | |
| Klenow(3′→5′exo-) | P7010-LC-L | 10,000 | 5,000U/ml | ProvenchoiceforDNAlabeling,A-tailing | |
| KlenowFragment | P7060L | 2,500 | 5,000U/ml | DNApolymeraseusefulforend-fillingpriortoblunt-endligation | |
| MakoDNAPolymerase | P7090L | 3,000 | 30,000U/ml | Exonuclease-deficientpolymerase,lacksstranddisplacementactivity | |
| Manta1.0DNAPolymerase | P7140-HC-L | 100,000 | 400,000U/ml | ThermostableBacillusDNAPolymerase,strongstranddisplacement | |
| (HighConcentration) | |||||
| Manta1.0DNAPolymerase | P7140-LC-L | 100,000 | 40,000U/ml | ThermostableBacillusDNAPolymerase,strongstranddisplacement | |
| (LowConcentration) | |||||
| Bitmap MMuLVReverseTranscriptase | P7040L | 100,000 | 200,000U/ml | Usefulpolymeraseforfirst-strandDNAsynthesis | |
| OmniKlentaq® | P7500-HC-L | 8,400 | 42,000U/ml | EngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes | |
| Bitmap (HighConcentration) | |||||
| OmniKlentaq® | P7500-LC-L | 8,400 | 4,200U/ml | Lowerconcentration–1microliterperreaction |

Concentration200,000U/mL
UnitSize10,000U
LotNumberShipmentdependent
StorageTemperature-25°Cto-15°C
SDSPurityn/a>99%
SpecificActivityn/a≥280,000U/mg
E.coliDNAContamination2,000<10copies
核酸内切酶
摘要:能够降解RNA/DNA杂交链中的RNA链。
大肠杆菌RNaseH(rnh)属于核酸内切酶,该酶能够降解RNA/DNA杂交链中的RNA链。该酶的降解产物核苷酸分子具有5-磷酸末端和3-羟基末端。该酶对于单链或双链RNA分子几乎无活性
产品组分
SuppliedWith
B9220(10XRNAseHBuffer)
10XRNAseHBuffer(B9220):
500mMTris-HCl
750mMKCl
30mMMgCl2
100mMDTT
pH8.3@25°C
产品信息:
PartNumberY9220L,Y9220F
Concentration5,000U/ml
UnitSize5,000U,770U
StorageTemperature-25to-15°C
Purity(SDS-PAGE)>99%
SpecificActivity625,000U/mg
E.coliDNAContamination500U<10copies
T4DNA聚合酶
摘要:为DNA克隆期间抛光5′和3′端提供可靠选择
T4DNA聚合酶能催化原始DNA模板以5′→3′方向延伸。该酶具有较强的3′→5′核酸外切酶活性,但缺乏内在的5′→3′核酸外切酶活性或链置换功能。

Suppliedin
100mMKPO4
1.0mMDTT
0.1mMEDTA
50%glycerol
pH6.5@25°C
SuppliedWith
B0110(10XBlueBuffer)
10XBlueBuffer(B0110)
500mMNaCl
100mMTris-HCl
100mMMgCl2
10mMDTT
pH7.9@25°C
PartNumberP7080L
Concentration3,000U/ml
UnitSize2,000U
StorageTemperature-15to-25°C
Purity(SDS-PAGE)>99%
SpecificActivity5,555U/mg
E.coliDNAContamination30U<10copies
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