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| ProductName | PartNo. | UnitSize | Concentration | Notes | |
| NEWPRODUCTS: | |||||
| E.coliDNALigase | L6090L | 2,500 | 10,000U/ml | EfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG | |
| T4RNALigase2 | L6080L | 4,500 | 30,000U/ml | LigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures | |
| T4RNALigase2Truncated | L6070L | 500 | 5,000U/ml | Requiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA | |
| OmniKlentaq® | P7500-HC-L | 8,400 | 42,000U/ml | EngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes | |
| （HighConcentration） | |||||
| OmniKlentaq® | P7500-LC-L | 8,400 | 4,200U/ml | Lowerconcentration–1microliterperreaction | |
| （LowConcentration） | |||||
| PhoenixHotStartTaqDNAPolymerase | P7590L | 500 | 5,000U/ml | AntibodybasedHotStartTaqDNApolymerasedeliversrobustPCRperformancewithexceptionalpre-PCRcyclingroomtemperaturestability | |
| VeraSeq™2.0High-FidelityDNAPolymerase | P7511L | 500 | 2,000U/ml | Industry-leadingspeed,fidelityandrobustnessdeliveredviaanengineeredPCRpolymerase | |
| VeraSeq™ULtraDNAPolymerase | P7520L | 500 | 2,000U/ml | Highfidelity,speed,androbustnessinPCRdeliveredbyanengineered,thermostableDNApolymerasethattoleratesuraciltemplates,nucleotidesandprimers | |
| RecA | Y9260L | 1.5mg | 2.0mg/ml | Promotesstrandexchangeofsingle-strandedDNAfragmentswithDNAduplexsubstrates | |
| ThermolabilePhosphatase | Y9210L | 5,500 | 5,000U/ml | Removes5′and3′-phosphategroupsfromDNA | |
| ThermolabileUDG | G5020L | 500 | 1,000U/ml | RemovesuracilfromDNA,leavingAPsite | |
| PolyAPolymerase | P7460L | 1,000 | 5,000U/ml | CatalyzestheadditionofAMPfromATPtothe3′hydroxylofRNAwhichisusefulformakingpolyAtailedRNA | |
| UltraPureLigases: | |||||
| Bitmap E.coliDNALigase | L6090L | 2,500 | 10,000U/ml | EfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG | |
| T3DNALigase | L6010L | 900,000 | 3,000,000U/ml | Worksinhighionicstrength–to1.0MNaCl | |
| T4DNALigase | L6030-LC-L | 150,000 | 120,000U/ml | Lowconcentrationovernightligationformat | |
| T4DNALigase（Rapid） | L6030-HC-L | 240,000 | 600,000U/ml | “Ultrapureligase”referencedinNatureMethods（Quail,1December2008） | |
| Bitmap T4RNALigase1 | L6050L | 10,000 | 20,000U/ml | Single-strandedRNAligase.Alsojoinssingle-strandedDNAmolecules | |
| T4RNALigase2 | L6080L | 4,500 | 30,000U/ml | LigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures | |
| Bitmap T4RNALigase2Truncated | L6070L | 500 | 5,000U/ml | Requiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA | |
| T7DNALigase | L6020L | 900,000 | 3,000,000U/ml | 1000foldhigheractivityonstickyendsthanbluntends | |
| TaqDNALigase | L6060L | 20,000 | 40,000U/ml | ThermostableDNAligasewhichefficientlysealsnicksanddiscriminatesagainstmismatchligation | |
| UltraPurePolymerases: | |||||
| DNAPolymeraseI | P7050L | 5,000 | 10,000U/ml | DNApolymerasewith5′→3′synthesis, | |
| 5′→3′and3′→5′exonucleaseactivities | |||||
| Klenow（3′→5′exo-） | P7010-HC-L | 10,000 | 50,000U/ml | ProvenchoiceforDNAlabeling,A-tailing | |
| Klenow（3′→5′exo-） | P7010-LC-L | 10,000 | 5,000U/ml | ProvenchoiceforDNAlabeling,A-tailing | |
| KlenowFragment | P7060L | 2,500 | 5,000U/ml | DNApolymeraseusefulforend-fillingpriortoblunt-endligation | |
| MakoDNAPolymerase | P7090L | 3,000 | 30,000U/ml | Exonuclease-deficientpolymerase,lacksstranddisplacementactivity | |
| Manta1.0DNAPolymerase | P7140-HC-L | 100,000 | 400,000U/ml | ThermostableBacillusDNAPolymerase,strongstranddisplacement | |
| （HighConcentration） | |||||
| Manta1.0DNAPolymerase | P7140-LC-L | 100,000 | 40,000U/ml | ThermostableBacillusDNAPolymerase,strongstranddisplacement | |
| （LowConcentration） | |||||
| Bitmap MMuLVReverseTranscriptase | P7040L | 100,000 | 200,000U/ml | Usefulpolymeraseforfirst-strandDNAsynthesis | |
| OmniKlentaq® | P7500-HC-L | 8,400 | 42,000U/ml | EngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes | |
| Bitmap （HighConcentration） | |||||
| OmniKlentaq® | P7500-LC-L | 8,400 | 4,200U/ml | Lowerconcentration–1microliterperreaction |
实时定量PCR 更好
ZipScript具有Enzymatics自己的RNA酶H负反转录酶（EnzScript）和快速激活的热启动DNA聚合酶（PhoenixHotStartTaq）混合物，可为用户提供非常敏感的单管RT-qPCR混合物。
货号：EnzymaticsY9040L
品名：T4PolynucleotideKinase（T4多核苷酸激酶）
产品描述：
T4多核苷酸激酶（PNK）催化ATP的末端γ位磷酸酯转移和交换到双链和单链DNA，RNA和核苷3-单磷酸酯分子的5-羟基末端（1）。T4PNK还表现出3-磷酸酶和2，3环磷酸二酯酶活性（2,3,4,5,6）。
规格： | 10,000U |
浓度： | 10,000U/ml |
保存： | -25°C至-15°C |
纯度（SDS-PAGE） | >99％ |
活性： | 133,333U/mg |
SS核酸外切酶 | 2000U<5.0%released |
DS核酸外切酶 | 2000U<1.0%released |
DS内切核酸酶 | 2000U=Noconversion |
大肠杆菌 DNA污染 | 2000U<10copies |
蛋白质来源
从表达重组T4多核苷酸激酶基因的大肠杆菌中纯化而得。
补充：
10mMTris-HCl
50mMKCl
0.1碌MATP
1.0mMDTT
0.1mMEDTA
50%glycerol
pH7.4@25掳C
随附：
B9040(10XT4PolynucleotideKinaseBuffer)：
10X多核苷酸激酶缓冲液（B9040）700mM
Tris-HCl
100mMMgCl 2
50mMDTT
pH7.6在25℃
单位定义
一个单位定义为在1XT4多核苷酸激酶反应缓冲液中，37℃下30分钟内催化掺入1nmol[ 32 P]（ATP供体）的酶量。
质量控制分析
单位表征测定
单位活性使用2倍系列稀释法测量。在1X反应缓冲液中制备酶的稀释液，并将其加入到50μL含有10μMOligodT单链DNA，1XPNK反应缓冲液和66μMATP的反应中，并将[γ- 32 P]ATP反应在37°孵育30分钟将其置于冰上，并使用Sambrook和Russell的方法（MolecularCloning，v3,2001，pp.A8.25-A8.26）进行分析。
蛋白质浓度（OD 280）测量使用使用2.0mg/mlBSA样品（PierceCat＃23209）标准化的NanodropND-1000分光光度计
在OD 280处分析2.0μL酶样品并用产品储存溶液冲切。使用消光系数63,440和分子量34,620道尔顿将观察到的3个重复样品的平均测量结果转换为mg/mL。
SDS-Page（物理纯度评估）
将2.0μL酶溶液加载到变性的4-20％Tris-甘氨酸SDS-PAGE凝胶上，其侧翼为宽范围MW标记和2.0μL1：100稀释的样品。电泳后，将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量，确认浓缩样品的纯度大于99％。
部分产品信息：
货号 | 品名 | 规格 | 浓度 | 品牌 |
|---|---|---|---|---|
N2070-10-L | ATPSolution | 50µmol | 10mM | Enzymatics |
N2050-10-L | 10mMdNTPSolutionMix | 100µmol | 10mM | Enzymatics |
N2010L | 100mM75µmoldNTPSolutionSet | 75µmol | 100mM | Enzymatics |
N2010-A-L | 100mMdATPSolution | 75µmol | 100mM | Enzymatics |
N2050L | 100µmoldNTPSolutionMix | 100µmol | 25mM | Enzymatics |
Y9180L | 10xUracilCleavageSystem | 750Reactions | N/A | Enzymatics |
P7670 | 2xHiFiPCRMasterMix | 1rxn | 2x | Enzymatics |
Y9420L | 5xER/A-TailingEnzymeMix | 24reactions | N/A | Enzymatics |
Y9380L | E.coli Pyrophosphatase | 50U | 100U/mL | Enzymatics |
Y9030L | E.coliSingle-StrandedDNABindingProtein | 1.5mg | 2.75mg/ml | Enzymatics |
Y9140-HC-L | End-RepairMix(HighConcentration) | 75Reactions | 1Rxn/µL | Enzymatics |
Y9140-LC-L | End-RepairMix(LowConcentration) | 200Reactions | 1Rxn/µL | Enzymatics |
Y9080L | EndonucleaseVIII | 10,000U | 10,000U/ml | Enzymatics |
P7600L | EnzScript™(MMLVReverseTranscriptaseRNaseH-) | 10,000U | 200,000U/mL | Enzymatics |
X8010L | ExonucleaseI | 30,000U | 20,000U/ml | Enzymatics |
X8020L | ExonucleaseIII | 50,000U | 100,000U/ml | Enzymatics |
P7010-HC-L | Klenow(3′→5′exo-)(HighConcentration) | 10,000U | 50,000U/ml | Enzymatics |
P7010-LC-L | Klenow(3′→5′exo-)(LowConcentration) | 10,000U | 5,000U/ml | Enzymatics |
P7060L | KlenowFragment | 2,500U | 5,000U/ml | Enzymatics |
X8030L | LambdaExonuclease | 10,000U | 5,000U/ml | Enzymatics |
P7040L | M-MuLVReverseTranscriptase | 100,000U | 200,000U/ml | Enzymatics |
P7090L | MakoDNAPolymerase(3→5exo-) | 3,000U | 30,000U/ml | Enzymatics |
P7140-HC-L | Manta1.0DNAPolymerase(HighConcentration) | 100,000U | 400,000U/ml | Enzymatics |
P7140-LC-L | Manta1.0DNAPolymerase(LowConcentration) | 100,000U | 40,000U/ml | Enzymatics |
P7610L | NGSLibraryPrep:2XVeraSeq™PCRMix–250Reactions | 250Reactions | 2X | Enzymatics |
P7590L | Phoenix™HotStartTaqDNAPolymerase | 500U | 5,000U/ml | Enzymatics |
P7460L | Poly(A)Polymerase | 1,000U | 5,000U/mL | Enzymatics |
Y9260L | RecA | 1.5mg | 2.0mg/mL | Enzymatics |
Y9220L | RNAseH | 5,000U | 5,000U/ml | Enzymatics |
Y9240L | RNAseInhibitor | 20,000U | 40,000U/ml | Enzymatics |
L6010L | T3DNALigase | 900,000U | 3,000,000U/ml | Enzymatics |
L6030-LC-L | T4DNALigase | 150,000U | 120,000U/ml | Enzymatics |
L6030-HC-L | T4DNALigase(Rapid) | 240,000U | 600,000U/ml | Enzymatics |
P7080L | T4DNAPolymerase | 2,000U | 3,000U/ml | Enzymatics |
Y9130L | T4Gene32Protein | 1.0mg | 10mg/mL | Enzymatics |
Y9040L | T4PolynucleotideKinase | 10,000U | 10,000U/ml | Enzymatics |
L6050L | T4RNALigase1 | 10,000U | 20,000U/ml | Enzymatics |
L6080L | T4RNALigase2 | 4,500U | 30,000U/ml | Enzymatics |
L6070L | T4RNALigase2Truncated | 500U | 5,000U/ml | Enzymatics |
L6020L | T7DNALigase | 900,000U | 3,000,000U/ml | Enzymatics |
P7260L | T7DNAPolymerase | 3,500U | 10,000U/ml | Enzymatics |
P7180L | T7RNAPolymerase | 50,000U | 50,000U/ml | Enzymatics |
L6060L | TaqDNALigase | 20,000U | 40,000U/ml | Enzymatics |
P7110L | TaqDSC2.0DNAPolymerase | 1,250U | 5,000U/ml | Enzymatics |
P7250L | Taq-BDNAPolymerase | 10,000U | 5,000U/ml | Enzymatics |
P7620L | TaqIT | 5,000U | 50,000U/mL | Enzymatics |
P7070L | TerminaldeoxynucleotidylTransferase(TdT) | 6,000U | 20,000U/ml | Enzymatics |
G5020L | ThermolabileUDG | 500U | 1,000U/ml | Enzymatics |
Y9370L | ThermostablePyrophosphatase | 1,250U | 2,000U/ml | Enzymatics |
G5010L | UracilDNAGlycosylase | 10,000U | 2,000U/ml | Enzymatics |
P7511L | VeraSeq™2.0High-FidelityDNAPolymerase | 500U | 2,000U/ml | Enzymatics |
P7520L | VeraSeq™ULtraDNAPolymerase | 500U | 2,000U/ml | Enzymatics |
L6030-W-L | WGSLigase | 24Reactions | N/A | Enzymatics |
P7640L | ZipScript™One-StepRT-qPCRKit | 1000Reactions | N/A | Enzymatics |
P7020-HC-L | φ29DNAPolymerase(HighConcentration) | 2,000U | 100,000U/ml | Enzymatics |
P7020-LC-L | φ29DNAPolymerase(LowConcentration) | 2,000U | 10,000U/ml | Enzymatics |
