EditPro™ Transfection Reagent历史发展

EditPro™ TransfectionReagenthasbeenoptimizedtodeliverCas9mRNAorRNPwiththeassociatedguideRNA, intoawiderangeofadherentcelltypes. Inaddition,ssDNAconstructsorsingle-strandedoligodeoxynucleotides(ssODN)canbemixedwiththeCas9mRNA/gRNAorCas9protein/gRNAtopromotehomology-directedrepair(HDR)tointroducetargetedsubstitutions. Itiswellsuitedforco-transfectiondeliveryofgeneeditingtools,therefore,intomanyprimarycellsandcelllines. 

HighEfficiencyCas9mRNADelivery

BelowwedemonstratetheefficiencyoftransfectionofCas9-encodingmodifiedmRNAfollowedbydetectionwithanti-Cas9antibodiesdemonstratinghighefficiencytransfectionandexpressionoftheCas9.

Figure1. AntibodylabelingofA549cellsandadulthumandermalfibroblasts24hourspost-transfectionconfirmedsuccessfultransfectionofCas9mRNAusingtheEditPro™TransfectionReagent.

Cas9-mediatedINDELFormation

ThefollowingdatainHeLacellsdemonstratessuccessfulgeneeditingwithEditPro™viaco-transfectionofeitherCas9mRNAorCas9proteinwiththeassociatedguideRNAsasindicatedbytheformationofINDELbands. 

Figure2. IndelanalysisfollowingtransfectionwithEditPro™TransfectionReagent.HeLacellsweretransfectedusing1or2µLofEditPro™TransfectionReagentasindicatedwithCas9mRNA(modified)/gBlock®U6sgRNAGAPDHoligo/GFPmRNA(modified)orCas9protein/gBlock®U6sgRNAGAPDHoligo/GFPmRNA(modified).Genome-modificationwasanalyzedusingtheT7Endo1assay.

Features:

• HighEfficiency–deliveringCas9RNAand/orCas9proteinforgeneeditingandDNAforhomology-directedrepair.

• Lowtoxicity 

• Easytouse–nospecialequipmentrequired

• CostEffective–effectiveatlowamountsofreagentresultinginlowcost/well