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Plantprobes/MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM20/LM20/1 Ea

(RatIgM)

SPECIFICITY:Theantibodyrecognisesthehomogalacturonandomainofpecticpolysaccharides.Ithasnoknowncross-reactivitywithotherpolymers.Itcanrecognisepecticpolysaccharidesinseveralspecies.Theantibodyrequiresmethyl-estersforrecognitionofhomogalacturonananddoesnotbindtoun-esterifiedhomogalacturonan.WenowrecommendtheuseofLM20intheplaceofJIM7asLM20bindingismoreeffectivelylostbyhighpHtreatmentsthatremoveHGmethylesters.

REFERENCES: Verhertbruggenetal.(2009)Carbohydr.Res.344,1858-1862.ONLINE

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