SignaGen转染试剂 for MPAC Cells

Description
GenJet™ DNA In Vitro Tranfection Reagent for M-PAC is pre-optimized for transfecting M-PAC (also celled mPAC) cells.  M-PAC is moderately impregnated pancreas acinar unit cells, also one of hard-to-transfect cell line.

Refer to the following optimal transfection conditions for maximal transfection efficiency on M-PAC cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency  Transfection Results: Reporter Gene Plasmid Efficiency (GFP %)

90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter)  60%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet 
 

温馨提示：不可用于临床治疗。