AMPK（A2/B1/G1）,active enzymes

货号：	P48-10H-10
保存条件：	–70
规格：	10ug

Description:Recombinantfull-lengthhumanAMPK(combinationofA2/B1/G1subunits)wasexpressedbybaculovirusinSf9insectcellsusingaC-terminalHistags.

 
    Species:Human

 
    Tag:HIStags

 
    ExpressionSystem:Sf9insectcellsusingbaculovirus

 
    Sequence:FullLength

 
    GenbankNumber:NM_006252 NM_006253 NM_002733

 
    
SpecificActivity:SampleKinaseActivityPlot.Forspecificinformationonagivenlot,seerelatedtechnicaldatasheet. 



Purity:SamplePurityData.Forspecificinformationonagivenlot,seerelatedtechnicaldatasheet. 



Storage,StABIlityandShipping:Storeproductat–70oC.Foroptimalstorage,aliquottargetintosmallerquantitiesaftercentrifugationandstoreatrecommendedtemperature.Formostfavorableperformance,avoidrepeatedhandlingandmultiplefreeze/thawcycles. 

Applications:KinaseAssay,WesternBlot 

MolecularWeight:~69kDa(A2),~38kDa(B1),and~40kDa(G1). 

GeneAliases:SubunitsA2:PRKAA2,AMPK,AMPK2,PRKAASubunitB1:PRKAB1,AMPK,HAMPKb,MGC17785SubunitG1:PRKAG1,AMPKG,MGC8666 

ScientificBackground:AMPK(A2/B1/G1)playsakeyroleininsulinsignalingpathwayandisamajortherapeutictargetforthetreatmentofdiabetes(1).AMPKisviewedasafuelsensorforglucoseandlipidmetabolismbymodulatingtheactivityoftheautonomousnervoussysteminvivo.Short-termoverexpressionofaconstitutivelyactiveformofAMPKintheliverleadstomildhypoglycemiaandfattyliverduetoincreasedfattyacidutilization(2). 

References: 
1.Viollet,B.etal:PhysiologicalroleofAMP-activatedproteinkinase(AMPK):insightsfromknockoutmousemodels.Biochem.Soc.Trans.2003;31;216–219. 

2.Foretz,M.etal:Short-termoverexpressionofaconstitutivelyactiveformofAMP-activatedproteinkinaseintheliverleadstomildhypoglycemiaandfattyliver.Diabetes,2005;54(5);1331-1339. 

Formulation:50mMsodiumphosphate,pH7.0,300mMNaCl,150mMimidazole,0.1mMPMSF,0.25mMDTT,25%glycerol.