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Usbio/024167 Claudin 1 (CLDN1) BioAssay™ ELISA Kit (Human)/024167/96Tests188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Usbio/024167 Claudin 1 (CLDN1) BioAssay™ ELISA Kit (Human)/024167/96Tests

Claudin 1 (CLDN1) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLDN1 in human tissue homogenates, cell lysates and other biological fluids.

Detection Range
31.2-2000pg/ml
Sensitivity
13.7pg/ml
Intra-Assay CV
<10%
Inter-Assay CV
<12%
Kit Components
*024167A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*024167B: Standard, 2x1vial024167C: Standard Diluent, 1x20ml*024167D: Detection Reagent A, 1x120ul*024167E: Detection Reagent B, 1x120ul024167F: Assay Diluent A, 1x12ml024167G: Assay Diluent B, 1x12ml024167H: TMB Substrate, 1x9ml024167K: Stop Solution, 1x6ml024167L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (024167K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *024167A, *024167B, *024167D and *024167E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
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