Usbio/pH-Regulated Antigen PRA1, Recombinant, Candida Albicans, aa16-299, His-Tag (PRA1)/10ug/518048

Cellsurfaceproteininvolvedinthehost-parasiteinteractionduringcandidalinfection.WithMP65,representsamajorcomponentofthebiofilmmatrix.Sequesterszincfromhosttissueandmediatesleukocyteadhesionandmigration.Asasurfaceprotein,bindsthetwohumancomplementregulatorsCFHandCFHR1,aswellasplasminogenPLG,mediatescomplementevasionandextra-cellularmatrixinteractionand/ordegradation.Asareleasedprotein,enhancescomplementcontrolindirectvicinityoftheyeastandthusgeneratesanadditionalprotectivelayerwhichcontrolshostcomplementattack,assistingthefungusinescapinghostsurveillance.Bindstohostfluid-phaseC3andblockscleavageofC3toC3aandC3b,leADIngtoinhibitionofcomplementactivation.MediatesalsohumancomplementcontrolandcomplementevasionthroughbindingtoC4BPA,anotherhumancomplementinhibitor,aswellasthroughbindingtohostintegrinalpha-M/beta-2.Decreasescomplement-mediatedadhesion,aswellasuptakeofC.albicansbyhumanmacrophages.||Source:|Recombinantproteincorrespondingtoaa16-299ofCandidaAlbicanspH-RegulatedAntigenPRA1,fusedto6xHis-TagatN-terminal,expressedinYeast.||MolecularWeight:|~33.4kD||AASequence:|APVTVTRFVDASPTGYDWRADWVKGFPIDSSCNATQYNQLSTGLQEAQLLAEHARDHTLRFGSKSPFFRKYFGNETASAEVVGHFDNVVGADKSSILFLCDDLDDKCKNDGWAGYWRGSNHSDQTIICDLSFVTRRYLTQLCSSGYTVSKSKTNIFWAGDLLHRFWHLKSIGQLVIEHYADTYEEVLELAQENSTYAVRNSNSLIYYALDVYAYDVTIPGEGCNGDGTSYKKSDFSSFEDSDSGSDSGASSTASSSHQHTDSNPSATTDANSHCHTHADGEVHC||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor6monthsafterreceiptat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Source:Recombinant,Yeast|Purity:≥90%(SDS-PAGE)|Concentration:Asreported|Form:SuppliedasaliquidinTris-HCl,pH8.0,1mMEDTA,50%glycerol.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.