Antibodies-Online/1Kb DNA Ladder/ABIN1540473/5 x 100 tests

Detection Range

500-10000 bp

Minimum Detection Limit

500 bp

Application

Agarose Gel Electrophoresis (AGE)

Options

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Purpose	1Kb DNA ladder (Quantitative) is ideal for determining the size of double-stranded DNA from 500 to 1000 base pairs.
Detection Method	Fluorometric
Characteristics	The ladder consists of 10 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 2000bp and 5000bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 2000bp and 5000bp are at 40ng. The 2000bp and 5000bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use. 10 DNA Fragments (bp): 500, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, and 10000 2000bp and 5000 Bands: 100ng/5 µL Other Bands: 40ng/5 µL Range: 500bp-10kb Bands: 10

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Application Notes	Recommended Loading: 5 µL/Lane. Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands. Recommended Loading: 5 µL/Lane, 8cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min.
Comment	Do not heat before loading. For quantification, adjust the concentration of the sample to equalize. Visualize DNA by staining with ethidium bromide or with SYBR Green I.
Restrictions	For Research Use only

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Format	Liquid
Storage	-20 °C
Storage Comment	1 year at room temperature, for long term storage, please keep at -20°C.
Expiry Date	12 months

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Supplier Images	1Kb DNA Ladder

友情链接：其他

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