Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Antibodies-Online/Ular UV-Induced DNA Damage ELISA Kit/ABIN2344977/96 tests188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

Antibodies-Online/Ular UV-Induced DNA Damage ELISA Kit/ABIN2344977/96 tests

Antigen
UlarUV-InducedDNADamage
Reactivity
Others
Alternatives
Kitswithalternativereactivityto:
1Others
MethodType
CellELISA
Application
ELISA
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeCellsarefirstseededina96-welltissuecultureplate.WellsarethenUVirrADIatedtoinduceDNAdamage.Afterfixationanddenaturation,cellscontainingCPDdamageareprobedwithananti-CPDantibody,followedbyanHRPconjugatedsecondaryantibody.Theunboundsecondaryantibodyisremovedduringawashstep,andsubstratesolutionreactivewithHRPisaddedtothewells.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.2
BrandOxiSelect™
SampleTypeCellSamples
AnalyticalMethodQuantitative
DetectionMethodColorimetric
CharacteristicsOxiSelect™CellularUV-inducedDNADamageELISAKit(CPD)isanenzymeimmunoassaydevelopedforrapiddetectionofCPDsingenomicDNAofculturedcells.Eachkitprovidessufficientreagentstoperformupto96assays.Figure1:StructuresofDNAlesionsinducedbyUVLight
Components
  1. Anti-CPDAntibody,100X:One100μLvial.
  2. SecondaryAntibody,HRPConjugate:One50μLvial.
  3. DenaturationSolutionA,100X:One200μLvial.
  4. DenaturationSolutionB,100X:One200μLvial.
  5. AssayDiluent:One50mLbottle.
  6. 10XWashBuffer:One50mLbottle.
  7. SubstrateSolution:One12mLamberbottle.
  8. StopSolution(Part.No.310808):One12mLbottle.
Materialnotincluded
  1. 96-welltissuecultureplate
  2. Celllineofinterest
  3. UVcrosslinker,irradiator,orgermicidallamp
  4. DPBScontainingmagnesiumandcalcium
  5. 75%Methanol/25%AceticAcid
  6. 70%Ethanol
  7. 10μLto1000μLadjustablesinglechannelmicroPipetteswithdisposabletips
  8. 50μLto300μLadjustablemultichannelmicropipettewithdisposabletips
  9. Microplatereadercapableofreadingat450nm(620nmasoptionalreferencewavelength)
BackgroundAbsorptionofultraviolet(UV)lightproducestwopredominanttypesofDNAdamage,cyclobutanepyrimidinedimers(CPD)andpyrimidine(6-4)pyrimidonephotoproducts(6-4PP)(Figure1).TheresultisatransitionofCtoTandCCtoTT,whicharethemostfrequentmutationsofp53inbothhumanandmouseskincancers.UVdamagedDNAisusuallyrepairedbynucleotideexcisionrepair(NER)orbaseexcisionrepair(BER).AfterUVexposure,cellsactivatep53andstallthecellcycleforrepair.Ifthedamageistoosevere,thecellwilltriggerapoptosistogetridofDNAdamaged,potentiallymutantcells.
Comment

  • MeasureCPDstructuresofDNAlesionsinducedbyUVlight
  • Intactcellsareculturedina96-wellplateandCPDmeasuredbyELISA

PlateWithoutplate
ReagentPreparation
  • 1XWashBuffer:Dilutethe10XWashBufferConcentrateto1Xwithdeionizedwater.Stirtohomogeneity.
  • Anti-CPDAntibodyandSecondaryAntibody,HRPConjugate:ImmediatelybeforeusedilutetheAnti-CPDAntibody1:100andSecondaryAntibody1:1000withAssayDiluent.Donotstoredilutedsolutions.
  • DenaturationSolutionA:ImmediatelybeforeusedilutetheDenaturationSolutionA1:100with70 %Ethanol.Donotstoredilutedsolution.
  • DenaturationSolutionB:ImmediatelybeforeusedilutetheDenaturationSolutionB1:100withDPBS(containingmagnesiumandcalcium).Donotstoredilutedsolution.
AssayProcedure

I.CellSeeding

  1. HarvestandresUSPendcellsinculturemediumat2-4x105cells/mL.Seed100μLineachwellofa96-welltissuecultureplateandincubateovernightat37 °C,5 %CO2(cellsshouldbe>80 %confluent).

II.UVTreatment,FixationandDenaturation

  1. Carefullyremovemediumfromthewellsbytiltingtheplateandaspiratingfromtheedge.Gentlyadd100μLofDPBS(containingmagnesiumandcalcium)toeachwell,takingcarenottodislodgethecells.
  2. PerformUVirradiationtodesiredwells(removalofplatecoverisrecommended).Includewellswithoutirradiationasanegativecontrol.Samplesshouldbeperformedintriplicate.
  3. Aspiratethewellsandadd100μLof75 %Methanol/25 %AceticAcidtoeachwell.Incubate30 minutesatroomtemperature.
  4. Aspiratethewellsandadd100μLof70 %Ethanoltoeachwell.Incubate30 minutesatroomtemperature.
  5. Aspiratethewellsandadd100μLofDenaturationSolutionA(seePreparationofReagents)toeachwell.Incubate5 minutesatroomtemperature.
  6. Gentlywash3timeswith200μLDPBS(containingmagnesiumandcalcium).
  7. Aspiratethewellsandadd100μLofDenaturationSolutionB(seePreparationofReagents)toeachwell.Incubate10 minutesatroomtemperature.
  8. Aspiratethewellsandadd200μLofAssayDiluenttoeachwell.Blockthewells30 minutesatroomtemperature.4

III.CPDDetection

  1. Aspiratethewellsandadd100μLofthedilutedanti-CPDantibody(seePreparationofReagents)toeachwell.Incubateatroomtemperaturefor1houronanorbitalshaker.
  2. Washmicrowellstrips4timeswith250μL1XWashBufferperwellwiththoroughaspirationbetweeneachwash.Afterthelastwash,emptywellsandtapmicrowellstripsonabsorbentpadorpapertoweltoremoveexcess1XWashBuffer.
  3. Add100μLofthedilutedSecondaryAntibody,HRPConjugate(seePreparationofReagents)toeachwell.Incubateatroomtemperaturefor1houronanorbitalshaker.
  4. Washmicrowellstrips4timesaccordingtostep2above.Proceedimmediatelytothenextstep.
  5. WarmSubstrateSolutiontoroomtemperature.Add100μLofSubstrateSolutiontoeachwell,includingtheblankwells.Incubateatroomtemperatureonanorbitalshaker.Actualincubationtimemayvaryfrom5-20 minutes.
  6. Stoptheenzymereactionbyadding100μLofStopSolutionintoeachwell,includingtheblankwells.Resultsshouldbereadimmediately(colorwillfadeovertime).
  7. Readabsorbanceofeachmicrowellonastandardmicroplatereaderusing450nmastheprimarywavelength.5

RestrictionsForResearchUseonly
Storage4°C
StorageCommentStoreallkitcomponentsat4°C.
SupplierImages
Immunofluorescence (IF) image for Ular UV-Induced DNA Damage ELISA Kit (ABIN2344977)DNADamageInducedbyUVLightinHelaCells.HeLacellswereseededat20K/wellover...
image for Ular UV-Induced DNA Damage ELISA Kit (ABIN2344977)DNADamageInducedbyUVLightinHeLaCells.HeLacellswereseededat20K/wellover...
Productcitedin:Dai,Zhou,Wei,Wang,Guo,Yi,Li,Gao,Liu,Li:"Afunctionalsingle-nucleotidepolymorphismintheERCC1genealterstheefficacyofnarrowbandultravioletBtherapyinpatientswithactivevitiligoinaChinesepopulation."in:TheBritishjournalofdermatology,Vol.173,Issue2,pp.457-63,2015(PubMed).

Harberts,Zhou,Fishelevich,Liu,Gaspari:"UltravioletradiationsignalingthroughTLR4/MyD88constrainsDNArepairandplaysaroleincutaneousimmunosuppression."in:Journalofimmunology(Baltimore,Md.:1950),Vol.194,Issue7,pp.3127-35,2015(PubMed).

Shin,Kum,Ryu,Kim,Jung,Park:"ProtectiveeffectsofanewphloretinderivativeagainstUVB-induceddamageinskincellmodelandhumanvolunteers."in:Internationaljournalofmolecularsciences,Vol.15,Issue10,pp.18919-40,2014(PubMed).

Emanuele,Bertona,Sanchis-Gomar,Pareja-Galeano,Lucia:"Protectiveeffectoftrehalose-loadedliposomesagainstUVB-inducedphotodamageinhumankeratinocytes."in:Biomedicalreports,Vol.2,Issue5,pp.755-759,2014(PubMed).

Thongrakard,Ruangrungsi,Ekkapongpisit,Isidoro,Tencomnao:"ProtectionfromUVBToxicityinHumanKeratinocytesbyThailandNativeHerbsExtracts."in:PhotochemistryandphotoBIOLOGy,2013(PubMed).

相关产品

matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY

intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15

goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP

novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg

novateinbio/SARS-Associated Coronavirus Spike Mosaic S ( C ) Recombinant ( SARS Mosaic S ( C ) )/500ug/PT_42025_500ug

matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB

intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24

goprolytix//10 ug, 100 ug/RTF-0300

最新签约

Liposoma

KAMIYA

Bioclone

intactgenomics

Abbexa

Emfret

Tribioscience

goprolytix

技术文章

ProChimia/HS(CH2)mCOOH/FT 002m101/1 g

Alomone经销商/代理商 Alomone蛋白质

prospec mSCF

【供应 GelRed核酸染料 （Biotium 41003）】价格_厂家 苏州蚂蚁淘

PDMS Device Fabrication and Surface Modification | Protocol

prospec HOR 250

上海正品NSJ Bioreagents生物试剂1 NSJ Bioreagents细胞生物学

杭州Enzymatics inc批发，产品货源 Enzymatics T4连接酶

新闻动态

苏州Enzymatics型号参数规格 Enzymatics超纯poly酶

19413561 GAMMASECRETASE INHIBITOR X 1MG

合肥中国Abbexa官网，Abbexa Ltd价格更新 abbexa授权代理

Abbexa中国采购 abbexa纯化蛋白

RhodamineDHPE美国/Biotium询价/索取资料——苏州蚂蚁淘

武汉美国Polysciences公司 分析试剂Polysciences

广州Anatrace型号参数规格 Anatrace膜蛋白提取与纯化

Genovis 特约代理苏州蚂蚁淘实验试剂有限公司

行业前沿

正品Pfenex生物试剂 重组白喉杆菌CRM197,为什么这么便宜?

PfenexX中国代理价格，厂家 SEA133鲑鱼纤维蛋白原,求推荐!

Pfenex中国采购 三文鱼凝血酶/鲑鱼凝血酶 Salmon Thrombin,是正品吗?

Polysciences聚乙烯亚胺 Polyethylenimine,好用吗?

PolysciencesX中国代理价格，厂家 Polysciences24765,哪里有卖?

Polysciences代理，产品目录 Polysciences聚合物,是正品吗?

Sea Run Holding产品促销 重组白喉杆菌CRM197,为什么这么便宜?

生命科学Pfenex价格优惠 Sea Run Holding代理，产品目录,有优惠活动吗?

最热产品

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003

Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003

Qubit™ dsDNA BR Assay Kit Q32853现货

Parafilm M封口膜/PM996/4x125/整箱

Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销

LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货

GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g

Leica徕卡819一次性窄刀片【50片/盒，10盒/条】/14035838925

推荐新闻

RA Biosources报价单 rabios代理

Lampire型号参数规格 Lampire公司

品牌试剂实验Medica Lisotopes代理 Medica Lisotopes产品

进口试剂QoolAbs直采 Qoolabs噬菌体筛选

进口Molecular Research Center品牌清关代理 MRC一步法TRIzol RNA抽提

授权代理商，正品 Spherefluidics授权

Ursabioscience美元价/进货价 Ursa BioScience产品

Wards Science抗体，试剂，血清，细胞 wardsci授权

新闻动态
行业前沿
技术文章
最新产品