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Antibodies-Online/Shisa Homolog 8 (Xenopus Laevis) (SHISA8) (AA 160-492) protein (His tag)/ABIN3076897/1 mg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Antibodies-Online/Shisa Homolog 8 (Xenopus Laevis) (SHISA8) (AA 160-492) protein (His tag)/ABIN3076897/1 mg

ProteinName
ShisaHomolog8(XenopusLaevis)(SHISA8)
  • C22orf17
  • Orf26
  • shisafamilymember8
  • SHISA8
ProteinCharacteristics
AA160-492
1AA39-492
Origin
Human
1Human
Source
InsectCells
1InsectCells
ProteinType
Recombinant
Purificationtag/Conjugate
ThisSHISA8proteinislabelledwithHistag.
Application
Crystallization(Crys),ELISA,SDS-PAGE(SDS),WesternBlotting(WB)
Options
Bulkdiscount
Supplier
SupplierProductNo.
SequenceRLGLERAHSPRARRTVTRALTELLKQPGPQEPLPPTLGPPLGGCVQVQMGDGLPRGSPHNSAGPRTPRLARASPPGEPFMRVAPPGLAAAAAARDSEPGPVPGAGGCGEDRLGSQPRPENGPPGSHRTASGRGLRRGDSEGGVNRGIGPSRVSLLAPDKKRLNNAPRGSAAPGPPRGPRLQGGGSLTLQPDYAKYATFKAAALKAAEAAPRDFCQRFPALEPSPRQPPARAPRPSPDLPAPLDACPWAPPVYAPPAAPGPYAAWTSSRPARPAPLSHPTARAFQVPRRPGHAARRQFSVKMPETFNPQLPGLYGSAGRGSRYLRTNSKTEVTV
Sequencewithouttag.Taglocationisatthediscretionofthemanufacturer.Ifyouhaveaspecialrequest,pleasecontactus.
Characteristics
  • MadeinGermany-fromdesigntoproduction-byhighlyexperiencedproteinexperts.
  • HumanSHISA8Protein(raisedinInsectCells)purifiedbymulti-step,protein-specificprocesstoensurecrystallizationgrade.
  • State-of-the-artalgorithmusedforplasmiddesign(Genesynthesis).
PurificationTwosteppurificationofproteinsexpressedinbaculovirusinfectedSF9insectcells:
  1. Inafirstpurificationstep,theproteinispurifiedfromtheclearedcelllysateusingthreedifferentHis-tagcapturematerials:highyield,EDTAresistant,orDTTresistant.EluatefractionsareanalyzedbySDS-PAGE.
  2. Proteincontainingfractionsofthebestpurificationaresubjectedtosecondpurificationstepthroughsizeexclusionchromatography.EluatefractionsareanalyzedbySDS-PAGEandWesternblot.
Purity>95 %asdeterminedbySDSPAGE,SizeExclusionChromatographyandWesternBlot.
Sterility0.22μmfiltered
EndotoxinLevelProteinisendotoxinfree.
GradeCrystallographygrade
MolecularWeight35.7kDaIncludingtag.
UniProtB8ZZ34
ApplicationNotesInadditiontotheapplicationslistedaboveweexpecttheproteintoworkforfunctionalstudiesaswell.Astheproteinhasnotbeentestedforfunctionalstudiesyetwecannotofferaguaranteethough.
Comment

Incasesinwhichitishighlylikelythattherecombinantproteinwiththedefaulttagwillbeinsolubleourproteinlabmaysuggestahighermolecularweighttag(e.g.GST-tag)insteadtoincreasesolubility.WewilldiscussallpossIBLeoptionswithyouindetailtoassurethatyoureceiveyourproteinofinterest.

RestrictionsForResearchUseonly
FormatLiquid
Buffer100mMNaCL,20mMHepes,10%glycerol.pHvalueisatthediscretionofthemanufacturer.
HandlingAdviceAvoidrepeatedfreeze-thawcycles.
Storage-80°C
StorageCommentStoreat-80°C.
ExpiryDateUnlimited(ifstoredproperly)
SupplierImages
image for Shisa Homolog 8 (Xenopus Laevis) (SHISA8) (AA 160-492) protein (His tag) (ABIN3076897)„CrystallographyGrade“proteinduetomulti-step,protein-specificpurificationprocess
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