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TheLEGENDplex™MouseAnti-VirusResponsePanelDetectionAntibodieswillbeusedtogetherwiththefollowingMixandMatchCaptureBeadsandMouseAnti-VirusResponsePanelStandard,andBufferSetItoofferflexIBLecustomizationwithinthepanel(13-plex): 740065LEGENDplex™MouseIFN-γCaptureBeadA4,13X740626LEGENDplex™MouseCXCL1(KC)CaptureBeadA5,13X740066LEGENDplex™MouseTNF-αCaptureBeadA6,13X740627LEGENDplex™MouseCCL2(MCP-1)CaptureBeadA7,13X740139LEGENDplex™MouseIL-12(p70)CaptureBeadA8,13X740628LEGENDplex™MouseCCL5(RANTES)CaptureBeadA10,13X740157 LEGENDplex™MouseIL-1βCaptureBeadB2,13X740630LEGENDplex™MouseCXCL10(IP-10)CaptureBeadB3,13X740631LEGENDplex™MouseGM-CSFCaptureBeadB4,13X740632LEGENDplex™MouseIL-10CaptureBeadB5,13X740145LEGENDplex™HumanMouseIFN-βCaptureBeadB6,13X740633LEGENDplex™MouseIFN-αCaptureBeadB7,13X740634LEGENDplex™MouseIL-6CaptureBeadB9,13X740624LEGENDplex™MouseAnti-VirusResponsePanelStandard740623LEGENDplex™BufferSetI(SetUpBeads,Buffers,PlateSealers,Matrix,SA-PE,andDataAnalysisSoftwareDongle)740377or740379FilterPlateorV-bottomPlateTheLEGENDplex™MouseAnti-VirusResponsePanelisabead-basedmultiplexassay,utilizingfluorescence–encodedbeadssuitableforuseonvariousflowcytometers.Thispanelallowssimultaneousquantificationof13mousecytokines,includinginterferons(α,β,γ),interleukins(1β,6,10,12),chemokines(CCL2(MCP-1),CCL5(RANTES),CXCL1(KC),CXCL10(IP-10)),TNF-α,andGM-CSF. ThisassaypanelprovideshigherdetectionsensitivitiesandbroaderdynamicrangesthantrADItionalELISAmethods.Thepanelhasbeenvalidatedforusewithserum,plasma,andcellculturesupernatants.
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