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MouseTumor-AssociatedEndothelialCells(HumanBreastCancerOrigin,MTMMECH)

CatalogNo.MC-6020h

SuggestedMedium: EndothelialCellMedium/wKit–500ml

CatalogNo.M1168

ProductDescription

MouseTumor-AssociatedEndothelialCells(HumanBreastCancerOrigin,MTMMECH)fromCellBIOLOGicswereisolatedfrompathogen-freelaboratorynudemice.MTMMEC.HfromCellBiologicswereisolatedfromhumanxenograftofpathogen-freelaboratorynudemice.Humanbreastadenocarcinomaepithelialcells(MDA-MB-231)weresubcutaneouslyinjectedintothenudemicefor4-6weeksandthetumortissueswerethenharvestedforcellculture.MTMMECHwereculturedinaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor14-35days.Cellsweredetachedfromflasksandimmediatelycryo-preservedinvials.Cellswillbedeliveredinafrozenvialandeachvialcontains0.5x10⁶cells/1ml.Themethodweusetoisolateprimarytumor-associatedendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

MTMMECHaretestedforexpressionofMarkersusingantibody,Ve-Cadherin(CD144,VE-CadherinAntibody,C-19,sc6458SantaCruz);orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.MTMMECHarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

MTMMECHcanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.

Primarycellsshouldneverbekeptat-20°C.

AuthorizedUsesofCellBiologics’Products

MTMMECHfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiABIlity

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.