Cell Biologics/Cynomolgus Monkey Primary Bladder Smooth Muscle Cells/MK-6205/

CynomolgusMonkeyPrimaryBladderSmoothMuscleCells

CatalogNo. MK-6205

SuggestedMedium: M2268SmoothMuscleCellMedium/wKit–500ML

ProductDescription

CynomolgusMonkeyPrimaryBladderSmoothMuscleCellsfromCellBIOLOGicsareisolatedfrom CynomolgusMonkey bladdersmoothmuscletissue.CynomolgusMonkeyPrimaryBladderSmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromthecultureflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶cellspermlandisdeliveredfrozen.Thecellsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.CynomolgusMonkeyPrimaryBladderSmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperiments(orcellsmaybeexpandedfor2passagesatasplitratioof1:2)underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

CynomolgusMonkeyPrimaryBladderSmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.

Nevercanprimarycellsbekeptat-20°C.

AuthorizedUsesofCellBiologics’Products

CynomolgusMonkeyPrimaryBladderSmoothMuscleCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughMonkeyPrimaryBladderSmoothMuscleCellsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiABIlity

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.