Cell Biologics/Canine Primary Pulmonary Artery Smooth Muscle Cells/D6083/

CaninePrimaryPulmonaryArterySmoothMuscleCells

CatalogNo. D-6083

SuggestedMedium: SmoothMuscleCellMedium/wKit–500ML

CatalogNo. M2268

ProductDescription

CaninePrimaryPulmonaryArterySmoothMuscleCellsfromCellBiologicsareisolatedfrompulmonaryarteryofbeagledog.CaninePrimaryPulmonaryArterySmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶cellspermlandisdeliveredfrozen.Thecellsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.CaninePrimaryPulmonaryArterySmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.CellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics(Cellsmaybeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics).Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

CaninePrimaryPulmonaryArterySmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologicsProducts

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.

Nevercanprimarycellsbekeptat-20°C.

AuthorizedUsesofCellBiologicsProducts

CaninePrimaryPulmonaryArterySmoothMuscleCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

AlthoughCaninePrimaryPulmonaryArterySmoothMuscleCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.

WarrantyandLiability

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.