Cell Biologics/ZDF Rat Diabetic Stomach Smooth Muscle Cells/RD6087/ T

ZDFRatDiabetic Stomach SmoothMuscleCells

CatalogNo. RD-6087

SuggestedMedium: SmoothMuscleCellMedium/wKit–500ml

CatalogNo. M2268

ProductDescription

ZDFRatDiabeticStomachSmoothMuscleCellsfrom CellBiologics areisolatedfrom stomach tissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶ cellspermlandisdeliveredfrozen.

ProductTesting

ZDFRatDiabetic Stomach SmoothMuscleCells arecharacterizedbyimmunofluorescentmethodwithantibodytoα-smoothmuscleactin. Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedby CellBiologics (Cellsmaybeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics). Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

ZDFRatDiabetic Stomach SmoothMuscleCells canbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

Storageof CellBiologics Products

CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.

AuthorizedUsesof CellBiologics’ Products

ZDFRatDiabetic Stomach SmoothMuscleCells from CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.

WarrantyandLiability

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.