Cell Biologics/Mouse Primary Diabetic Liver Epithelial Cells/MD6044/ Fr

MousePrimaryDiabetic LiverEpithelialCells

CatalogNo.  MD-6044

SuggestedMedium:EpithelialCellMedium/wKit–500ml

CatalogNo. M6621

ProductDescription

MousePrimaryDiabeticLiverEpithelialCellsfrom CellBiologics areisolatedfromtheliverofDiabetic(db/db)mice.MousePrimaryDiabeticLiverEpithelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶cellsperml.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.CellsareincubatedwithEpCAM-1(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

MousePrimaryDiabeticLiverEpithelialCellsarecharacterizedbyimmunofluorescentstainingwithantibodiesofE-cadherin(Catalog610182,BD),ZO-1(Catalog617300,LifeTechnology).Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

MousePrimaryDiabeticLiverEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

Storageof CellBiologics’ Products

CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C

AuthorizedUsesof CellBiologics’ Products

MousePrimaryDiabeticLiverEpithelialCellsfrom CellBiologics aredistributedfor internal laboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiability

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ Cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.