Cell Biologics/Human Primary Brain Microvascular Endothelial Cells/H6023/

HumanPrimaryBrainMicrovascularEndothelialCells

CatalogNo. H-6023

SuggestedMedium: CompleteHumanEndothelialCellMedium–500ML

CatalogNo. H1168

ProductDescription

HumanPrimaryBrainMicrovascularEndothelialCellsfromCellBiologicsareisolatedfromhumanbraintissue.HumanPrimaryBrainMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryopreservedinvails.Eachvialcontainsatleast0.5×10⁶ cellsperml. Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

HumanPrimaryBrainMicrovascularEndothelialCellsfromCellBiologicsdisplaytypicalcobblestonewithlargedarknucleiappearanceunderlightmicroscopy.CellsaretestedforexpressionofendothelialcellMarkerusingantibody,CD31(CatalogNo.550389,BD;CD31/PECAM-1PE-conjugatedAntibody,CatalogNo.FAB3567P,R&D)orVE-Cadherin(FITC-VE-cadherinCatalogNo.560411,BD)byimmunofluorescencestainingorFACS.Allcellstestnegativeformycoplasma,bacteria,yeast,andfungi.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.Perrequest,aCertificateofAnalysiswillbeprovidedforeachcelllotpurchased.Cellscanbeexpandedfor3-5passagesunderthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

HumanPrimaryBrainMicrovascularEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.

Nevercanprimarycellsbekeptat-20°C.

AuthorizedUsesofCellBiologics’Products

HumanPrimaryBrainMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.

CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughHumanPrimaryBrainMicrovascularEndothelialCellsareisolatedfromnormalhumantissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiability

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ humanendothelialcellswith CellBiologics’ Media(catalognumberH1168) andCoatingSolutionforappropriateendothelialcellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.