Cell Biologics/Mouse Primary Diabetic Bone MarrowDerived Endothelial Cells/MD6031/

MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCells

CatalogNo. MD-6031

SuggestedMedium:EndothelialCellMedium/wKit–500ml

CatalogNo. M1168

ProductDescription

MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCellsfromCellBiologicsareisolatedfromtheBoneMarrowofDiabetic(db/db)mice.MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryopreservedinvials.Eachvialcontainsatleast0.5×10⁶cellsperml.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCellsaretestedforexpressionofMarkersusingantibody,VE-cadherin(CD144,VE-cadherinAntibody,C-19,sc6458,SantaCruz);AF1002(R&DSystem)orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.Cellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C.

AuthorizedUsesofCellBiologics’Products

MousePrimaryDiabeticBoneMarrow-DerivedEndothelialCellsfromCellBiologicsaredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiability

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’CellswithCellBiologics’Media(catalognumberM1168)andCoatingSolutionforappropriateendothelialcellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.