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| Cat-Nr. | M10-063S |
| Size | 5 µg |
| Price | 75 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 145 |
| Molecular Weight | 16.6 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED50 for this effect is ≤ 15 µg/ml. |
| Species Reactivity | Mouse |
| Buffer | 5mM Tris, pH 7.6 |
| Reconstitution | Centrifuge the vial prior to opening Reconstitute in 5mM Tris, pH 7.6 to 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | apm-1 |
| Description | gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. Recombinant mouse gAcrp30 is a 16.6 kDa protein consisting of 145 amino acid residues. |
| Protein Sequence | MKGEPGEAAY MYRSAFSVGL ETRVTVPNVP IRFTKIFYNQ QNHYDGSTGK FYCNIPGLYY FSYHITVYMK DVKVSLFKKD KAVLFTYDQY QEKNVDQASG SVLLHLEVGD QVWLQVYGDG DHNGLYADNV NDSTFTGFLL YHDTN |
| Uniprot ID | Q60994 |
| Protein RefSeq | NP_033735.3 |
| mRNA RefSeq | NM_009605.4 |
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